Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P3T49_RS00580 Genome accession   NZ_CP120723
Coordinates   105957..107336 (+) Length   459 a.a.
NCBI ID   WP_094231234.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain NX-12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100957..112336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T49_RS00560 ctsR 101311..101775 (+) 465 WP_003328320.1 transcriptional regulator CtsR -
  P3T49_RS00565 - 101789..102346 (+) 558 WP_003328319.1 UvrB/UvrC motif-containing protein -
  P3T49_RS00570 - 102346..103437 (+) 1092 WP_010787416.1 protein arginine kinase -
  P3T49_RS00575 clpC 103434..105872 (+) 2439 WP_003328317.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  P3T49_RS00580 radA 105957..107336 (+) 1380 WP_094231234.1 DNA repair protein RadA Machinery gene
  P3T49_RS00585 disA 107340..108422 (+) 1083 WP_010787418.1 DNA integrity scanning diadenylate cyclase DisA -
  P3T49_RS00590 - 108543..109643 (+) 1101 WP_061570389.1 PIN/TRAM domain-containing protein -
  P3T49_RS00595 ispD 109657..110355 (+) 699 WP_268372145.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P3T49_RS00600 ispF 110348..110824 (+) 477 WP_268508189.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49590.99 Da        Isoelectric Point: 8.2443

>NTDB_id=808329 P3T49_RS00580 WP_094231234.1 105957..107336(+) (radA) [Bacillus atrophaeus strain NX-12]
MAKSKTKFICQSCGYESAKWMGKCPGCGAWNTMTEETVKKTPANRRAAFAHSNQTVKKPSPITSIETSEEPRIKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSTHSVLYISGEESVKQTKLRADRLGINNQTLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVSVSIASSFRDTPPNPADCIIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=808329 P3T49_RS00580 WP_094231234.1 105957..107336(+) (radA) [Bacillus atrophaeus strain NX-12]
ATGGCTAAATCAAAAACGAAATTTATCTGCCAATCCTGCGGCTATGAATCCGCAAAGTGGATGGGGAAATGTCCGGGCTG
CGGAGCTTGGAACACAATGACAGAAGAAACAGTAAAAAAAACACCGGCTAATCGCAGAGCAGCTTTTGCACACTCTAACC
AAACCGTAAAGAAACCTTCACCTATCACATCAATAGAAACATCTGAAGAACCCCGAATTAAAACACAGCTTGGAGAATTT
AACAGAGTGCTGGGCGGCGGTGTTGTGAAGGGCTCACTTGTCTTAATTGGAGGAGATCCCGGTATTGGTAAGTCAACCTT
GCTGCTTCAGGTATCCGCTCAGTTATCAGACTCCACGCATAGTGTGCTCTACATTTCAGGCGAGGAATCTGTGAAACAAA
CGAAACTCAGAGCAGATCGGCTCGGTATTAATAACCAAACGTTACATGTTTTATCTGAAACCGACATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCATTTGTGGTTGTTGACTCCATTCAGACGGTCTATCAAAGCGATATTACATCAGC
TCCGGGCAGTGTGTCCCAAGTCAGGGAGTGTACGGCAGAGCTGATGAGAATTGCCAAGACAAAGGGTATACCGATTTTTA
TCGTAGGACATGTAACAAAAGAAGGTTCAATTGCTGGTCCGAGACTGTTGGAACATATGGTTGATACTGTTCTTTACTTT
GAAGGAGAGCGGCATCATACGTTCCGCATCCTGCGGGCAGTAAAAAACCGTTTCGGCTCTACAAATGAAATGGGCATTTT
TGAAATGCGGGAAGAGGGTCTGACAGAGGTGCTGAATCCTTCTGAAATCTTTCTTGAGGAACGATCAGCCGGAGCGGCAG
GTTCCAGCATAGTAGCGTCTATGGAAGGCACCAGACCGATTCTCGTGGAAATACAGGCCCTTATCTCACCGACCAGCTTT
GGGAATCCCCGGCGTATGGCCACGGGAATTGACCATAACAGAGTGTCACTGCTGATGGCTGTGTTAGAAAAAAGAGTCGG
GCTGCTGCTTCAAAATCAAGATGCGTATTTAAAGGTAGCAGGCGGCGTTAAGCTGGATGAACCGGCAATCGATCTCGCTG
TATCTGTCAGTATTGCATCAAGCTTTAGGGATACACCGCCAAATCCTGCCGACTGCATTATAGGAGAAGTCGGTTTAACA
GGTGAGGTCCGCAGGGTATCCAGAATTGAACAGCGTGTGAAAGAAGCTGCTAAACTTGGATTTAAGCGTATGATCATACC
TGCGGCGAATTTAGATGGATGGACAAAACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(69-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

94.541

99.782

0.943
  radA Streptococcus mitis NCTC 12261

63.135

98.693

0.623
  radA Streptococcus pneumoniae Rx1

63.135

98.693

0.623
  radA Streptococcus pneumoniae D39

63.135

98.693

0.623
  radA Streptococcus pneumoniae R6

63.135

98.693

0.623
  radA Streptococcus pneumoniae TIGR4

63.135

98.693

0.623
  radA Streptococcus mitis SK321

62.914

98.693

0.621