Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   OO986_RS01615 Genome accession   NZ_AP026552
Coordinates   333600..335804 (-) Length   734 a.a.
NCBI ID   WP_108694712.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328600..340804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS01595 (VV208B2_02880) hslU 329634..330965 (-) 1332 WP_011079331.1 HslU--HslV peptidase ATPase subunit -
  OO986_RS01600 (VV208B2_02890) hslV 330993..331547 (-) 555 WP_011079332.1 ATP-dependent protease subunit HslV -
  OO986_RS01605 (VV208B2_02900) - 331697..332254 (-) 558 WP_011151294.1 SPOR domain-containing protein -
  OO986_RS01610 (VV208B2_02910) cytR 332364..333371 (-) 1008 WP_011079334.1 DNA-binding transcriptional regulator CytR Regulator
  OO986_RS01615 (VV208B2_02920) priA 333600..335804 (-) 2205 WP_108694712.1 primosomal protein N' Machinery gene
  OO986_RS01620 (VV208B2_02930) rpmE 336085..336306 (+) 222 WP_011079336.1 50S ribosomal protein L31 -
  OO986_RS01625 (VV208B2_02940) - 336585..337862 (+) 1278 WP_011079337.1 malic enzyme-like NAD(P)-binding protein -
  OO986_RS01630 (VV208B2_02950) metJ 337949..338266 (-) 318 WP_011079338.1 met regulon transcriptional regulator MetJ -
  OO986_RS01635 (VV208B2_02960) - 338478..339644 (+) 1167 WP_013571024.1 O-succinylhomoserine (thiol)-lyase -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 81953.54 Da        Isoelectric Point: 8.5772

>NTDB_id=80824 OO986_RS01615 WP_108694712.1 333600..335804(-) (priA) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPIKAVLDHRPIWPDSVYS
LLLWCSHFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQAFLDE
EVASTTLRTLEEKGWISCEEKTPQLARWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTALFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=80824 OO986_RS01615 WP_108694712.1 333600..335804(-) (priA) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGCGTCCATCCATTGCTCGTGTTGCGCTGCCAGTGCCACTCGATAAACAATTTGATTATCTCGTTCCTGGACACCTTTT
TCCGATCATTGGTGGTCGCGTGAGTGTGCCATTTGGACGACAAACCTTGATTGGCATTGTCACCGCTTTGGTTCAAGAAT
CAGACTTTCCACGCGAGCAACTTAAACCCATTAAAGCGGTGTTGGATCATCGTCCTATTTGGCCCGATTCCGTCTACAGT
TTATTACTTTGGTGTAGCCACTTTTACCAATATCCCTTAGGTGAAACACTGCATAACGCCCTCCCCGCGGCACTAAGAAA
AGGCAAAGCGGCTGACTTTGCCACTCTACAAGAATGGCAACTGACCGAGTTAGGCAAAAACCAGTTGATGCAAGGGTTAA
ACAGTCGTGCGATAAAGCAAGCGAAAGTATTAAATATGCTTGCTAATGGCCCTCTGCCTCACCAAGCCTTTTTGGATGAG
GAAGTGGCATCCACAACCTTACGCACCTTAGAAGAAAAAGGCTGGATCAGCTGTGAAGAAAAAACGCCACAGTTGGCTCG
TTGGGGGAATCCGATTGAACATCCCGTCGATAAGCCGAAACTCAACCAAGAGCAAGCCATTGCCATTGCGACCGTTAACA
GCCAGACAGACTTTGGCTGTTATCTGTTGGAAGGGGTCACAGGTTCAGGCAAAACCGAAGTTTATCTCAACTTGATCAAA
CCCATTTTGGAACAAGGCAAACAGGCTCTGGTGTTGGTCCCTGAAATTGGTTTAACACCACAAACCATCAATCGTTTTCG
TCAACGGTTTGATGTGCCAGTCGCCGTCATCCATTCTGGTCTTAATGATACTGAACGTCTCAACGCTTGGCTTTCCGCAC
GAGATAAAGCGGCGGGCATTGTCATTGGTACACGCTCTGCACTACTGATCCCATTTGCTCAATTGGGGATCATCATTGTC
GATGAAGAGCACGACGCTTCGTACAAACAGCAAGATAGCTTGCGTTACCATGCTCGCGATGTGGCTGTGATGCGAGCGGC
AAAAGAAGGCATTCCAATTGTTCTCGGTTCAGCCACGCCCGCGTTAGAAACCTTACACAACGCACTTACGGGCAAATATC
ATCATCTCTCGCTAACCCAACGAGCAGGAGTGGCCGTGCCAACCACCAACAAAGTGCTGGATGTCAAAGGGCTCTATCTG
GAAAGCGGCCTATCAGCGCCACTCATTGCTGAAATGCGTCGTCATTTAAAAGCGGGCAATCAGGTGATGCTGTTTCTCAA
CCGTCGAGGCTTCTCCCCTGCGTTAATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGCTGCGATGCCTATTACA
CTTATCACCAGTACAGCAATGAAATCCGCTGTCACCACTGTGGTTCTCAACAACCTATTATCCATCAATGCCAAGGTTGT
GGTTCGACACAACTGGTGACGGTTGGTGTCGGCACTGAGCAACTGGAAAACCAGTTGACGGCCCTGTTTCCCGATTACAA
AGCGATTCGCATCGACCGCGATAGCACACGACGCAAAGGCAGCCTCGAAAGCGCTTTAGAAGCCATTCGCAAAGGGGAAT
ACCAAATTTTAATTGGCACGCAAATGCTGGCTAAAGGGCATCATTTCCCTGACGTGACGTTAGTGGCACTGTTGGATGTG
GATGGGTCACTGTACAGCAGTGATTTCCGAGCGTCCGAGCGATTGGCTCAGTTGTTTATTCAAGTCGCAGGACGAGCAGG
GCGTGCCAGCAAACCTGGCGAAGTGATATTACAAACCCACCATCCAGAACACACCTTGCTCAAAGCTCTGTTGACCAAGG
ATTACCGGCATTTTGCGCAGTCTGCACTGGAAGAACGGAAAATTGCCCAACTTCCCCCGTTTAGTTTTCTAACGCTATTT
CGCGCAGAAGCCAATCACAGCGAAATCGTCGAAACATTCTTACGCCAAGTGCGTATGACTCTCGAGTCTCATCCCTTGTT
TGATCACACTTGTCTCGTGCTCGGTCCAACCCCCGCTCCACTGGCCAAACGTGCGGGCAAATACCGCTGGCAGCTTCTGC
TTCAAACTCAACATCGTTCCTTGATGCAGAAAATACTCTCGAGCACGAAACCTGCTATCGATTTGTTACCAGATGCGAAA
AAAGTACGTTGGAATTTGGATATTGAGCCACAGGATCTGAGTTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(205-364)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

Helicase conserved C-terminal domain (Helicase_C)

(514-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.342

100

0.435

Multiple sequence alignment