NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	P4B08_RS25155	Genome accession	NZ_CP120704
Coordinates	5423359..5423811 (-)	Length	150 a.a.
NCBI ID	WP_028681203.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain SE5356
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5418359..5428811

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B08_RS25135 (P4B08_25145)	ampD	5419054..5419620 (-)	567	WP_003094640.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
P4B08_RS25140 (P4B08_25150)	-	5419771..5422026 (-)	2256	WP_016264271.1	DUF1631 domain-containing protein	-
P4B08_RS25145 (P4B08_25155)	nadC	5422312..5423160 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
P4B08_RS25155 (P4B08_25165)	pilA	5423359..5423811 (-)	453	WP_028681203.1	pilin	Machinery gene
P4B08_RS25160 (P4B08_25170)	pilB	5424043..5425743 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
P4B08_RS25165 (P4B08_25175)	pilC	5425747..5426967 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
P4B08_RS25170 (P4B08_25180)	pilD	5426971..5427843 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
P4B08_RS25175 (P4B08_25185)	coaE	5427840..5428451 (+)	612	WP_179063299.1	dephospho-CoA kinase	-
P4B08_RS25180 (P4B08_25190)	yacG	5428448..5428648 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15671.99 Da Isoelectric Point: 7.7813

                                >NTDB_id=807970 P4B08_RS25155 WP_028681203.1 5423359..5423811(-) (pilA) [Pseudomonas aeruginosa strain SE5356]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV

GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=807970 P4B08_RS25155 WP_028681203.1 5423359..5423811(-) (pilA) [Pseudomonas aeruginosa strain SE5356]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA

GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC

TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT

GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	65.789	100	0.667
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	45	100	0.48
pilA	Vibrio cholerae strain A1552	45	100	0.48
pilA	Vibrio cholerae C6706	45	100	0.48
pilA/pilAI	Pseudomonas stutzeri DSM 10701	46	100	0.46
pilA	Acinetobacter baumannii strain A118	45.27	98.667	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	42.568	98.667	0.42
comP	Acinetobacter baylyi ADP1	41.333	100	0.413
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.958	95.333	0.4
pilA2	Legionella pneumophila strain ERS1305867	38.926	99.333	0.387
pilA2	Legionella pneumophila str. Paris	38.255	99.333	0.38
pilA	Acinetobacter nosocomialis M2	42.537	89.333	0.38
pilA	Ralstonia pseudosolanacearum GMI1000	39.583	96	0.38

Detailed information NTG0807970