Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RI132_RS01640 Genome accession   NZ_CP134783
Coordinates   330720..332183 (-) Length   487 a.a.
NCBI ID   WP_039552533.1    Uniprot ID   -
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325720..337183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS01610 (RI132_01610) pmbA 325791..327134 (+) 1344 WP_011078776.1 metalloprotease PmbA -
  RI132_RS01615 (RI132_01615) mgtE 327236..328591 (-) 1356 WP_011078775.1 magnesium transporter -
  RI132_RS01620 (RI132_01620) - 328806..329081 (-) 276 WP_011078774.1 HPr family phosphocarrier protein -
  RI132_RS01625 (RI132_01625) rapZ 329084..329947 (-) 864 WP_011078773.1 RNase adapter RapZ -
  RI132_RS01630 (RI132_01630) ptsN 329960..330406 (-) 447 WP_103194324.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RI132_RS01635 (RI132_01635) hpf 330409..330696 (-) 288 WP_011078771.1 ribosome hibernation promoting factor -
  RI132_RS01640 (RI132_01640) rpoN/rpoN1 330720..332183 (-) 1464 WP_039552533.1 RNA polymerase factor sigma-54 Machinery gene
  RI132_RS01645 (RI132_01645) lptB 332237..332962 (-) 726 WP_011078769.1 LPS export ABC transporter ATP-binding protein -
  RI132_RS01650 (RI132_01650) lptA 332962..333456 (-) 495 WP_011078768.1 lipopolysaccharide transport periplasmic protein LptA -
  RI132_RS01655 (RI132_01655) lptC 333437..334000 (-) 564 WP_011078767.1 LPS export ABC transporter periplasmic protein LptC -
  RI132_RS01660 (RI132_01660) kdsC 333997..334554 (-) 558 WP_176301094.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RI132_RS01665 (RI132_01665) kdsD 334554..335525 (-) 972 WP_011149382.1 arabinose-5-phosphate isomerase KdsD -
  RI132_RS01670 (RI132_01670) - 335547..336512 (-) 966 WP_011078764.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54639.62 Da        Isoelectric Point: 4.3763

>NTDB_id=807863 RI132_RS01640 WP_039552533.1 330720..332183(-) (rpoN/rpoN1) [Vibrio vulnificus strain GCU-01]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEEHQEEAQLNGEDKAINEGSDSDDFSVEIDI
PDSSDVIEKSEISSELEIDTTWDDVYSANSGNTGIALDDDMPVYQGETTETLHDYLQWQLDLTPFSDTDRMIAFAIIDAV
DDTGYLTISPEEILESLNSVDIELDEVEAVRKRIQQFDPLGVASQNLQECLLLQLATFPIETPWLAEAKMVLSHHIDALG
NRDYKLIVKEAKLKEEDLRETLKLIQQLDPRPGSKINAIEPEYVIPDVSVFKEHGKWSVAINPDSIPKLKVNQQYAQLGK
GSSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQHDFFEYGEEAMKPMVLNDVATAVEMHESTISRVTTQKFM
HTPRGIFELKYFFSSHVSTDSGGECSSTAIRALIKKLVTAENSAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=807863 RI132_RS01640 WP_039552533.1 330720..332183(-) (rpoN/rpoN1) [Vibrio vulnificus strain GCU-01]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCTCAGCTCCAGCAGGCCATACGTTTGCTGCA
GCTGTCCACCTTGGATTTACAGCAAGAAATCCAAGAAGCGCTGGAATCAAATCCCTTATTGGAAGTGGAAGAGCATCAAG
AAGAAGCGCAACTGAATGGCGAAGACAAAGCGATCAATGAAGGTTCCGATTCTGATGATTTTTCTGTTGAGATCGACATT
CCTGACAGCTCTGATGTGATTGAAAAATCCGAGATCAGTAGCGAACTGGAAATCGACACCACCTGGGATGACGTTTACAG
TGCCAACTCTGGCAATACGGGCATCGCACTCGACGATGACATGCCGGTCTATCAAGGTGAAACGACGGAAACACTGCACG
ACTACTTACAGTGGCAACTGGATTTAACCCCGTTTAGCGATACTGACCGCATGATCGCCTTTGCCATCATTGATGCCGTC
GACGATACTGGCTACCTCACTATCAGCCCTGAAGAGATCTTAGAAAGCCTGAACAGCGTCGATATCGAACTCGACGAAGT
GGAAGCCGTACGCAAACGCATCCAACAGTTCGATCCCCTTGGTGTCGCGTCGCAAAACCTACAAGAATGCTTGCTGCTAC
AACTGGCCACATTTCCTATCGAGACCCCTTGGCTAGCCGAAGCCAAAATGGTCCTGAGCCATCACATTGATGCGCTAGGC
AATCGCGATTACAAGCTGATCGTCAAAGAAGCGAAGCTTAAAGAAGAAGACCTACGTGAAACGCTCAAACTGATCCAGCA
ACTTGATCCAAGGCCTGGCAGTAAGATCAATGCCATCGAGCCTGAGTACGTGATACCAGATGTATCCGTATTTAAAGAGC
ATGGCAAATGGAGTGTCGCGATCAATCCAGACAGCATTCCAAAATTAAAAGTAAATCAACAGTATGCGCAACTAGGCAAA
GGCAGCAGCGCCGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCGAAATGGTTAATTAAGAGCCTTGAGAGCAGAAA
CGAGACGCTGCTCAAAGTTGCACGTTGCATAGTTGAACATCAACATGATTTCTTCGAGTATGGGGAAGAAGCAATGAAAC
CTATGGTGCTAAATGATGTGGCAACCGCGGTTGAGATGCATGAATCCACCATCTCACGCGTGACAACCCAGAAGTTTATG
CACACGCCTAGAGGTATCTTTGAATTGAAATATTTCTTCTCTAGCCATGTTAGTACGGATAGTGGAGGAGAATGTTCATC
GACAGCAATTCGCGCACTCATTAAGAAGTTAGTCACTGCCGAAAACAGTGCGAAACCACTGAGTGATAGCAAAATTGCTG
CCTTACTGGCAGACCAAGGAATCCAGGTCGCACGGCGAACGATAGCTAAGTACCGCGAGTCCTTAGGCATTGCCCCATCT
AGTCAGCGAAAACGCCTACTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.366

100

0.45