Detailed information NTG0807647

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: P3X63_RS06290
• Genome accession: NZ_CP120679
• Coordinates: 1234140..1237832 (+)
• Length: 1230 a.a.
• NCBI ID: WP_077737082.1
• Uniprot ID: A0A6I7F6A0
• Organism: Bacillus sp. HSf4
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1229140..1242832

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3X63_RS06280 (P3X63_06280)	modB	1229886..1230542 (+)	657	WP_026586327.1	molybdate ABC transporter permease subunit	-
P3X63_RS06285 (P3X63_06285)	addB	1230653..1234180 (+)	3528	WP_026586328.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
P3X63_RS06290 (P3X63_06290)	addA	1234140..1237832 (+)	3693	WP_077737082.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
P3X63_RS06295 (P3X63_06295)	sbcD	1237890..1239071 (+)	1182	WP_026586329.1	exonuclease subunit SbcD	-
P3X63_RS06300 (P3X63_06300)	sbcC	1239068..1242466 (+)	3399	WP_277692609.1	exonuclease subunit SbcC	-
P3X63_RS06305 (P3X63_06305)	-	1242520..1242738 (-)	219	WP_026586331.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 140738.45 Da
• Isoelectric Point: 5.5682

>NTDB_id=807647 P3X63_RS06290 WP_077737082.1 1234140..1237832(+) (addA) [Bacillus sp. HSf4]
MDMTKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITKKENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKEIAENPGSLHLRRQLALLNRASISTLHSFCLQVIRKYYYLIDIDPAFRIADQTEGELLGDEVLDELFEDEYKKGDPA
FFELVDRYTTDRHDLDLQYLVKRVYEFSRSHPDPEGWLITFADLYDAGQDTGVESLPFYPYIKEDIALVLEGAREKLARA
LELAKQPGGPAPRAENFADDLAQIDRLIEHKDDFSALYELVPAVSFKRAKPCKGDEFEPALIDEATELRNGAKKQLEKLK
SDYFARTPDQHLTDLKAMKPVIDTLVKLVLEYGKRYKAAKQEKAIVDFSDLEHDCLAILAQKNEAGETVPSEAAKHYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTEDGSGPGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEETKTELVIIEQQEQAADEEAEELETVQLEARAMAGQIRKL
IAEKFQVYDGKTKTTRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEAAEVSVTLSLLKVIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIQDKKATFYEAMKAYLALADRNDSLYDKLRRFEGLLKKWRVFAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGIGRSFNMMDLNKPYLLDKELGFGTKFIHPKWRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLYLLG
SAKDKEKLLAKWRTSAARPGWLLPDFDRFQAKSYLDFVGPAVVRHRDMAGVGLDSADDEIRHHPSRFQISWLSAADLQAE
NGKQAGDEKQERLAQIQMGQPINARFDYQKEVGERLTWSYPHQDAAKIRTKQSVSEIKRQKEYEDEYGDRSLIRQTNESH
LFRRPSFMMAKGLTAAERGTAMHTVMQHIPLSKAPEQDELSLFLDRLVQHELLTEEQRAAIDEDDILAFFETEIGEKLLG
ARRVEREVPFNMTLPAGEVYPELQTADEPVLIQGIIDCLFETADGFYLLDYKTDRIQGKYANGMADAEPALRKRYETQIQ
LYSRAVETMLKTPLAGRALYFFDGGHVLKF

Nucleotide

• Length: 3693 bp

>NTDB_id=807647 P3X63_RS06290 WP_077737082.1 1234140..1237832(+) (addA) [Bacillus sp. HSf4]
GTGGACATGACGAAGCCAAAGGGCAGCACATGGACAGATGATCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATATT
AGTTGCCGCAGCGGCGGGGTCCGGAAAAACGGCCGTTCTTGTCGAACGGATCATCCGAAAAATTACGAAAAAGGAAAACC
CGATTGATGTGGACAGGCTGCTTGTCGTGACCTTTACGAATGCATCGGCGGCAGAGATGAAGCATCGGATCGGTGAAGCG
CTTGAAAAAGAGATTGCCGAAAACCCCGGCTCCCTTCACTTGCGCAGACAGCTCGCTTTATTGAACAGGGCCAGCATCTC
AACACTGCATTCATTCTGCCTGCAGGTCATTCGCAAATATTATTATCTGATCGACATCGATCCGGCTTTTCGGATCGCCG
ATCAGACCGAAGGCGAACTGCTTGGCGATGAGGTGCTTGATGAGCTGTTTGAAGATGAATATAAAAAAGGAGATCCCGCC
TTTTTCGAACTTGTGGATCGATATACGACAGACCGCCATGATCTTGATTTGCAGTATCTTGTGAAGCGCGTCTATGAATT
TTCACGGTCACATCCCGATCCGGAAGGCTGGCTTATAACATTTGCCGACCTTTATGACGCCGGTCAAGACACGGGTGTGG
AGTCACTGCCGTTCTATCCTTATATTAAGGAAGATATCGCGCTCGTACTTGAAGGGGCGCGGGAAAAGCTTGCGCGTGCG
CTGGAGCTGGCCAAACAGCCGGGCGGACCCGCTCCCCGCGCCGAAAACTTCGCGGACGATTTGGCGCAGATCGATAGGCT
GATCGAGCACAAGGATGACTTTTCCGCCCTTTATGAGCTTGTTCCCGCTGTATCGTTCAAAAGGGCAAAACCGTGCAAAG
GAGACGAGTTTGAGCCCGCATTAATCGATGAAGCGACAGAGCTCCGCAACGGTGCGAAAAAACAGCTGGAAAAACTGAAA
AGCGATTATTTTGCCCGGACCCCTGATCAGCATTTGACTGACCTGAAGGCGATGAAGCCTGTCATTGATACGCTTGTCAA
GCTTGTTCTGGAATATGGAAAGCGCTATAAGGCGGCGAAGCAGGAAAAGGCGATCGTGGATTTCTCTGATCTTGAGCACG
ATTGCCTGGCGATTTTGGCGCAAAAAAACGAAGCCGGCGAAACAGTGCCGAGTGAAGCGGCGAAGCATTACCGGCACCAG
TTCCATGAGGTGCTTGTCGATGAGTACCAAGACACCAACCTTGTGCAGGAAGCGATTTTAAAGCTGGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAACAGTCGATTTACCGCTTTCGCCTCGCAGAACCGCTTTTGTTTC
TCTCGAAATATAAACGGTTTACAGAGGACGGCTCAGGCCCGGGCCAAAAAATCGATTTGAACAAAAACTTCCGCAGCCGT
TCAGACATATTGGACAGCACCAATTTCTTGTTTAAGCAGCTGATGGGGGAAAAAGTCGGTGAAGTGGAATATGACGAACA
AGCCGAATTGAAGCTCGGCGCATCTTACCCTCCGAATGAAGAAACAAAAACAGAGCTTGTGATCATCGAACAACAGGAAC
AAGCCGCCGATGAAGAAGCGGAGGAGCTTGAAACGGTTCAGCTGGAAGCGCGGGCCATGGCCGGACAGATCCGCAAACTG
ATTGCGGAGAAATTTCAGGTCTATGACGGCAAAACGAAAACGACCCGCAATATCCAATACCGCGATATTGTTGTTTTGCT
GCGGTCCATGCCATGGGCGCCGCAGATTATGGACGAGTTCAAACAGCAGGGGATTCCCGTTTATGCGAATTTGTCGACAG
GCTATTTTGAAGCGGCTGAAGTTTCCGTCACGCTTTCATTATTAAAAGTCATTGACAATGCGTACCAGGACATCCCGCTC
GCCTCTGTGCTCAGATCTCCTGTCGTCGGCCTTGATGAAAATGAGCTTTCCCTGATCAGGATTCAGGACAAGAAAGCGAC
GTTCTACGAAGCGATGAAGGCTTACCTCGCGCTTGCCGACCGCAATGACAGCCTTTACGATAAGCTCCGCCGCTTTGAAG
GGCTTCTGAAAAAATGGAGAGTGTTTGCGAAAAACCACTCGGTAGCTGAGCTGATTTGGGAAATTTACCGCGATACGAAA
TACTTGGATTATGTTGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAACCTGCGCGCGCTTTACGACCGGGCGAGATC
ATATGAAGCGACTTCATTCCGCGGCCTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGCGGCGATGACCTCG
GAACGGCAAGAGCTCTCAGCGAACAGGAGGATGTCGTGCGGCTGATGACGATCCATTCAAGCAAAGGGCTGGAATTTCCT
GTCGTGTTCACGGCAGGGATCGGCCGGAGCTTTAATATGATGGATCTGAACAAACCGTATCTGCTCGATAAAGAGCTCGG
CTTCGGCACGAAATTCATCCATCCAAAATGGCGGATCAGCTATCCGACGCTGCCTCTTGTGGCGATGAAGAAAAAAATGC
GCCGCGAACTTTTGTCAGAGGAACTGCGCGTCTTGTACGTCGCACTGACACGTGCGAAGGAAAAGCTTTATTTGCTCGGA
TCGGCAAAAGATAAGGAAAAGCTCTTGGCGAAATGGCGAACTTCGGCTGCGCGGCCGGGATGGCTGCTGCCGGACTTTGA
CCGCTTTCAGGCCAAATCTTACTTGGATTTTGTCGGTCCGGCCGTAGTGCGTCACAGGGATATGGCAGGTGTTGGCCTCG
ATTCGGCGGATGACGAAATCCGGCATCACCCGTCCCGCTTTCAGATCAGCTGGCTCTCCGCCGCTGACCTTCAAGCCGAA
AACGGTAAACAAGCCGGGGACGAAAAACAGGAAAGGCTGGCCCAAATTCAAATGGGACAGCCGATCAATGCCCGGTTTGA
CTATCAAAAAGAAGTCGGAGAACGGCTGACATGGTCATATCCGCATCAAGACGCAGCGAAAATCCGCACCAAACAGTCTG
TTTCCGAAATCAAGAGGCAAAAGGAATATGAGGACGAGTACGGTGACCGTTCATTGATCCGCCAGACAAATGAGTCACAC
CTGTTCAGACGGCCATCCTTTATGATGGCGAAGGGACTAACCGCGGCCGAACGCGGAACGGCGATGCACACCGTCATGCA
GCACATCCCGCTTTCGAAAGCTCCGGAACAAGACGAGCTCAGCCTCTTTCTCGACAGGCTTGTTCAACATGAGCTTTTAA
CAGAAGAGCAGCGCGCTGCAATCGATGAAGACGATATTCTCGCGTTTTTCGAAACGGAGATCGGTGAAAAGCTCCTCGGC
GCCCGCCGGGTGGAGCGTGAAGTTCCCTTTAATATGACGCTTCCGGCCGGAGAGGTTTATCCCGAATTGCAGACAGCGGA
CGAACCCGTGTTGATTCAGGGAATAATCGACTGTTTGTTTGAGACGGCTGACGGGTTTTATTTGCTCGATTACAAAACAG
ACCGGATACAAGGGAAATACGCAAACGGCATGGCCGACGCCGAGCCGGCTTTAAGGAAACGCTATGAAACTCAGATTCAA
CTTTATTCACGCGCTGTTGAAACAATGCTCAAGACCCCGCTTGCAGGCCGCGCCCTGTACTTTTTTGACGGCGGGCATGT
ACTCAAGTTTTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-879)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1050-1222)

3D structure

• AlphaFold DB: A0A6I7F6A0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	72.602	100	0.726