Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P3T70_RS01575 Genome accession   NZ_CP120668
Coordinates   326207..327580 (+) Length   457 a.a.
NCBI ID   WP_277705546.1    Uniprot ID   -
Organism   Limosilactobacillus fermentum strain CYLB55     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 321207..332580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T70_RS01545 (P3T70_01545) - 321211..322086 (+) 876 WP_023465750.1 alpha/beta hydrolase -
  P3T70_RS01550 (P3T70_01550) - 322403..323740 (-) 1338 WP_277705541.1 C1 family peptidase -
  P3T70_RS01555 (P3T70_01555) - 323804..324418 (-) 615 WP_277705542.1 sugar O-acetyltransferase -
  P3T70_RS01560 (P3T70_01560) rpiA 324446..325132 (-) 687 WP_277705543.1 ribose-5-phosphate isomerase RpiA -
  P3T70_RS01565 (P3T70_01565) - 325144..325455 (-) 312 WP_277705544.1 GNAT family N-acetyltransferase -
  P3T70_RS01570 (P3T70_01570) - 325655..326194 (+) 540 WP_277705545.1 dUTP diphosphatase -
  P3T70_RS01575 (P3T70_01575) radA 326207..327580 (+) 1374 WP_277705546.1 DNA repair protein RadA Machinery gene
  P3T70_RS01580 (P3T70_01580) - 327613..328746 (+) 1134 WP_021815665.1 PIN/TRAM domain-containing protein -
  P3T70_RS01585 (P3T70_01585) gltX 328817..330313 (+) 1497 WP_012390831.1 glutamate--tRNA ligase -
  P3T70_RS01590 (P3T70_01590) - 330381..330950 (+) 570 WP_004562737.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49323.75 Da        Isoelectric Point: 8.9999

>NTDB_id=807556 P3T70_RS01575 WP_277705546.1 326207..327580(+) (radA) [Limosilactobacillus fermentum strain CYLB55]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLGVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=807556 P3T70_RS01575 WP_277705546.1 326207..327580(+) (radA) [Limosilactobacillus fermentum strain CYLB55]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTTCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGGAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGGCGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGAGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACAGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

68.433

99.125

0.678
  radA Streptococcus mitis NCTC 12261

68.212

99.125

0.676
  radA Streptococcus pneumoniae Rx1

68.212

99.125

0.676
  radA Streptococcus pneumoniae D39

68.212

99.125

0.676
  radA Streptococcus pneumoniae R6

68.212

99.125

0.676
  radA Streptococcus pneumoniae TIGR4

68.212

99.125

0.676
  radA Bacillus subtilis subsp. subtilis str. 168

63.895

100

0.639