Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RM375_RS20010 Genome accession   NZ_CP134715
Coordinates   4134414..4134854 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain RIVM_C040753     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4129414..4139854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RM375_RS19990 (RM375_19990) aroP 4130414..4131784 (+) 1371 WP_000399019.1 aromatic amino acid transporter AroP -
  RM375_RS19995 (RM375_19995) ampE 4131827..4132681 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  RM375_RS20000 (RM375_20000) ampD 4132678..4133229 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RM375_RS20005 (RM375_20005) nadC 4133317..4134210 (+) 894 WP_001135161.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RM375_RS20010 (RM375_20010) pilA 4134414..4134854 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  RM375_RS20015 (RM375_20015) pilB 4134864..4136249 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  RM375_RS20020 (RM375_20020) hofC 4136239..4137441 (+) 1203 WP_000157289.1 protein transport protein HofC -
  RM375_RS20025 (RM375_20025) guaC 4137476..4138519 (-) 1044 WP_001217338.1 GMP reductase -
  RM375_RS20030 (RM375_20030) - 4138675..4138719 (-) 45 WP_120795372.1 protein YacM -
  RM375_RS20035 (RM375_20035) coaE 4138744..4139364 (+) 621 WP_001269522.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=807556 RM375_RS20010 WP_000360914.1 4134414..4134854(+) (pilA) [Escherichia coli strain RIVM_C040753]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=807556 RM375_RS20010 WP_000360914.1 4134414..4134854(+) (pilA) [Escherichia coli strain RIVM_C040753]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363