Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RM375_RS10465 Genome accession   NZ_CP134715
Coordinates   2226246..2227256 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain RIVM_C040753     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2221246..2232256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RM375_RS10435 (RM375_10435) nudB 2222967..2223419 (+) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -
  RM375_RS10440 (RM375_10440) yebC 2223448..2224188 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RM375_RS10445 (RM375_10445) ruvC 2224223..2224744 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RM375_RS10450 (RM375_10450) yebB 2224746..2225348 (-) 603 WP_001024942.1 YebB family permuted papain-like enzyme -
  RM375_RS10455 (RM375_10455) yobI 2225422..2225487 (+) 66 WP_072123646.1 stress response small protein YobI -
  RM375_RS10460 (RM375_10460) ruvA 2225626..2226237 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RM375_RS10465 (RM375_10465) ruvB 2226246..2227256 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RM375_RS10470 (RM375_10470) znuB 2227404..2228189 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  RM375_RS10475 (RM375_10475) znuC 2228186..2228941 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  RM375_RS10480 (RM375_10480) znuA 2229020..2229952 (+) 933 WP_001339711.1 zinc ABC transporter substrate-binding protein ZnuA -
  RM375_RS10485 (RM375_10485) mepM 2229968..2231290 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=807536 RM375_RS10465 WP_000568522.1 2226246..2227256(+) (ruvB) [Escherichia coli strain RIVM_C040753]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=807536 RM375_RS10465 WP_000568522.1 2226246..2227256(+) (ruvB) [Escherichia coli strain RIVM_C040753]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494