Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P5623_RS03170 Genome accession   NZ_CP120625
Coordinates   509245..510315 (+) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain DSM 103869     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504245..515315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5623_RS03135 (P5623_03135) - 505496..505810 (+) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -
  P5623_RS03140 (P5623_03140) - 505835..506008 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  P5623_RS03145 (P5623_03145) - 506169..506807 (+) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  P5623_RS03150 (P5623_03150) - 506856..507098 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  P5623_RS03155 (P5623_03155) - 507311..507691 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  P5623_RS03165 (P5623_03165) - 507917..508789 (+) 873 WP_035390819.1 STAS domain-containing protein -
  P5623_RS03170 (P5623_03170) comGA 509245..510315 (+) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  P5623_RS03175 (P5623_03175) comGB 510296..511336 (+) 1041 WP_268414585.1 competence type IV pilus assembly protein ComGB -
  P5623_RS03180 (P5623_03180) comGC 511353..511646 (+) 294 WP_057079311.1 competence type IV pilus major pilin ComGC Machinery gene
  P5623_RS03185 (P5623_03185) comGD 511639..512085 (+) 447 WP_277687665.1 competence type IV pilus minor pilin ComGD -
  P5623_RS03190 (P5623_03190) comGE 512069..512383 (+) 315 WP_058214150.1 competence type IV pilus minor pilin ComGE -
  P5623_RS03195 (P5623_03195) comGF 512325..512822 (+) 498 WP_268414587.1 competence type IV pilus minor pilin ComGF -
  P5623_RS03200 (P5623_03200) comGG 512819..513202 (+) 384 WP_024718845.1 competence type IV pilus minor pilin ComGG -
  P5623_RS03205 (P5623_03205) - 513263..513458 (+) 196 Protein_528 YqzE family protein -
  P5623_RS03210 (P5623_03210) - 513493..513807 (-) 315 WP_236781621.1 DUF3889 domain-containing protein -
  P5623_RS03215 (P5623_03215) tapA 514078..514605 (+) 528 WP_277687666.1 amyloid fiber anchoring/assembly protein TapA -
  P5623_RS03220 (P5623_03220) - 514656..515228 (+) 573 WP_017357979.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=807183 P5623_RS03170 WP_017357987.1 509245..510315(+) (comGA) [Bacillus altitudinis strain DSM 103869]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=807183 P5623_RS03170 WP_017357987.1 509245..510315(+) (comGA) [Bacillus altitudinis strain DSM 103869]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAGGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTACAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGAGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649