Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5657_RS01235 Genome accession   NZ_CP120621
Coordinates   243029..243478 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain DSM 13019     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 238029..248478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5657_RS01215 (P5657_01215) hxlR 239241..239603 (-) 363 WP_163117762.1 transcriptional activator HxlR -
  P5657_RS01220 (P5657_01220) hxlA 239835..240467 (+) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  P5657_RS01225 (P5657_01225) hxlB 240473..241030 (+) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  P5657_RS01230 (P5657_01230) tlpC 241140..242861 (+) 1722 WP_021481821.1 methyl-accepting chemotaxis protein TlpC -
  P5657_RS01235 (P5657_01235) nucA/comI 243029..243478 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  P5657_RS01240 (P5657_01240) nin/comJ 243505..243903 (+) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  P5657_RS01245 (P5657_01245) bglC 243940..245373 (-) 1434 WP_123373936.1 6-phospho-beta-glucosidase -
  P5657_RS01250 (P5657_01250) - 245445..246173 (-) 729 WP_021481826.1 AAA family ATPase -
  P5657_RS01255 (P5657_01255) - 246192..246533 (-) 342 WP_213419533.1 DUF3147 family protein -
  P5657_RS01260 (P5657_01260) - 246521..246943 (-) 423 WP_213419590.1 MarR family transcriptional regulator -
  P5657_RS01265 (P5657_01265) yckD 247071..247403 (-) 333 WP_032724908.1 YckD family protein -
  P5657_RS01270 (P5657_01270) yckC 247485..247940 (-) 456 WP_024572178.1 RDD family protein -
  P5657_RS01275 (P5657_01275) - 247960..248149 (-) 190 Protein_245 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=807017 P5657_RS01235 WP_014475770.1 243029..243478(+) (nucA/comI) [Bacillus subtilis strain DSM 13019]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=807017 P5657_RS01235 WP_014475770.1 243029..243478(+) (nucA/comI) [Bacillus subtilis strain DSM 13019]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987