Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5653_RS10490 Genome accession   NZ_CP120610
Coordinates   2028750..2029178 (+) Length   142 a.a.
NCBI ID   WP_020451590.1    Uniprot ID   A0A6N2GRD1
Organism   Bacillus paralicheniformis strain DSM 28591     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2023750..2034178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5653_RS10460 (P5653_10460) - 2024006..2024614 (+) 609 WP_023857599.1 flavin reductase family protein -
  P5653_RS10465 (P5653_10465) wrbA 2024816..2025430 (+) 615 WP_026579653.1 NAD(P)H:quinone oxidoreductase -
  P5653_RS10470 (P5653_10470) - 2025741..2025881 (+) 141 WP_023857597.1 hypothetical protein -
  P5653_RS10475 (P5653_10475) - 2025874..2026707 (+) 834 WP_020451587.1 prohibitin family protein -
  P5653_RS10480 (P5653_10480) - 2026796..2027557 (+) 762 WP_026579652.1 hypothetical protein -
  P5653_RS10485 (P5653_10485) - 2027617..2028456 (+) 840 WP_026579651.1 thymidylate synthase -
  P5653_RS10490 (P5653_10490) nucA/comI 2028750..2029178 (+) 429 WP_020451590.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P5653_RS10495 (P5653_10495) lexA 2029377..2030000 (-) 624 WP_020451591.1 transcriptional repressor LexA -
  P5653_RS10500 (P5653_10500) yneA 2030152..2030463 (+) 312 WP_020451592.1 cell division suppressor protein YneA -
  P5653_RS10505 (P5653_10505) - 2030482..2031129 (+) 648 WP_020451593.1 recombinase family protein -
  P5653_RS10510 (P5653_10510) - 2031195..2031428 (+) 234 WP_020451594.1 DUF896 domain-containing protein -
  P5653_RS10515 (P5653_10515) tkt 2031592..2033595 (+) 2004 WP_023857593.1 transketolase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15420.36 Da        Isoelectric Point: 7.8950

>NTDB_id=806829 P5653_RS10490 WP_020451590.1 2028750..2029178(+) (nucA/comI) [Bacillus paralicheniformis strain DSM 28591]
MIKKWAVHLLFSALVLLGLSGGAAYSPQHAEGAARYDDVLYFPASRYPETGAHISDAIKAGHSDVCTIERSGADKRRQES
LKGIPTKPGFDRDEWPMAMCEEGGKGASVRYVSSSDNRGAGSWVGNRLSDYADGTRILFIVR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=806829 P5653_RS10490 WP_020451590.1 2028750..2029178(+) (nucA/comI) [Bacillus paralicheniformis strain DSM 28591]
ATGATCAAAAAATGGGCGGTTCATCTGCTGTTTTCCGCATTGGTGCTGCTTGGGCTTTCGGGAGGGGCTGCATATTCTCC
TCAGCATGCCGAAGGCGCTGCAAGGTATGATGACGTATTGTATTTTCCGGCATCGCGCTATCCTGAAACAGGTGCTCATA
TAAGCGATGCGATCAAAGCGGGCCATTCAGATGTCTGCACAATTGAAAGATCTGGAGCGGATAAGCGCCGTCAGGAATCA
TTAAAGGGGATTCCGACTAAGCCGGGCTTTGACCGTGACGAATGGCCGATGGCCATGTGTGAAGAAGGGGGAAAAGGAGC
GTCGGTCAGATATGTCAGCTCATCGGATAACCGCGGAGCCGGCTCCTGGGTCGGGAACAGGCTGAGCGATTACGCTGACG
GGACGAGAATTTTGTTTATCGTTCGATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N2GRD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.524

88.732

0.528