Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P5636_RS08900 Genome accession   NZ_CP120609
Coordinates   1632501..1633553 (-) Length   350 a.a.
NCBI ID   WP_013085018.1    Uniprot ID   D5DMH8
Organism   Priestia megaterium DSM 319     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1627501..1638553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5636_RS08860 (P5636_08860) - 1628518..1629342 (+) 825 WP_013085011.1 YqhG family protein -
  P5636_RS08865 (P5636_08865) - 1629366..1629550 (-) 185 Protein_1766 YqzE family protein -
  P5636_RS08870 (P5636_08870) comGG 1629594..1629968 (-) 375 WP_042452721.1 competence type IV pilus minor pilin ComGG -
  P5636_RS08875 (P5636_08875) comGF 1629958..1630395 (-) 438 WP_013085013.1 competence type IV pilus minor pilin ComGF -
  P5636_RS08880 (P5636_08880) - 1630392..1630730 (-) 339 WP_013085014.1 type II secretion system protein -
  P5636_RS08885 (P5636_08885) comGD 1630714..1631196 (-) 483 WP_218039677.1 competence type IV pilus minor pilin ComGD -
  P5636_RS08890 (P5636_08890) comGC 1631138..1631449 (-) 312 WP_013085016.1 competence type IV pilus major pilin ComGC -
  P5636_RS08895 (P5636_08895) comGB 1631464..1632510 (-) 1047 WP_013085017.1 competence type IV pilus assembly protein ComGB -
  P5636_RS08900 (P5636_08900) comGA 1632501..1633553 (-) 1053 WP_013085018.1 competence type IV pilus ATPase ComGA Machinery gene
  P5636_RS08905 (P5636_08905) - 1633823..1634206 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  P5636_RS08910 (P5636_08910) - 1634469..1634705 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  P5636_RS08915 (P5636_08915) - 1634743..1635375 (-) 633 WP_013085020.1 MBL fold metallo-hydrolase -
  P5636_RS08920 (P5636_08920) - 1635584..1635760 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  P5636_RS08925 (P5636_08925) - 1635796..1636698 (-) 903 WP_013085021.1 M14 family metallocarboxypeptidase -
  P5636_RS08930 (P5636_08930) - 1636810..1637778 (-) 969 WP_013085022.1 ROK family glucokinase -
  P5636_RS08935 (P5636_08935) - 1637781..1637987 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39671.03 Da        Isoelectric Point: 9.1859

>NTDB_id=806755 P5636_RS08900 WP_013085018.1 1632501..1633553(-) (comGA) [Priestia megaterium DSM 319]
MTEIEQFATYVIRDAYECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=806755 P5636_RS08900 WP_013085018.1 1632501..1633553(-) (comGA) [Priestia megaterium DSM 319]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATATGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACATTAGAAGATCCTATTGAAAAACCGAGCGAAACCGTCCTTCAAGTACA
GGTAAATGAACGGGCAGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGCGTTAGCTTTCAAGAAATGGAACAGACCCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGAA
AACATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCACTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5DMH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.857

100

0.589