Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5636_RS02715 Genome accession   NZ_CP120609
Coordinates   492497..492919 (+) Length   140 a.a.
NCBI ID   WP_013083984.1    Uniprot ID   A0A1Q8UP88
Organism   Priestia megaterium DSM 319     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 487497..497919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5636_RS02680 (P5636_02680) - 487827..488660 (-) 834 WP_013083979.1 AraC family transcriptional regulator -
  P5636_RS02685 (P5636_02685) - 488883..489122 (+) 240 WP_277735918.1 hypothetical protein -
  P5636_RS02690 (P5636_02690) - 489157..489243 (+) 87 Protein_537 two-component sensor histidine kinase -
  P5636_RS02695 (P5636_02695) - 489244..489738 (+) 495 WP_277735920.1 ATP-binding protein -
  P5636_RS02700 (P5636_02700) - 489707..490348 (+) 642 WP_013083981.1 response regulator transcription factor -
  P5636_RS02705 (P5636_02705) - 490480..491862 (+) 1383 WP_013083982.1 PepSY domain-containing protein -
  P5636_RS02710 (P5636_02710) - 491892..492356 (+) 465 WP_042452390.1 FixH family protein -
  P5636_RS02715 (P5636_02715) nucA/comI 492497..492919 (+) 423 WP_013083984.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P5636_RS02720 (P5636_02720) - 493074..493427 (+) 354 WP_258468631.1 DUF1516 family protein -
  P5636_RS02725 (P5636_02725) - 493468..494061 (-) 594 WP_013083986.1 hypothetical protein -
  P5636_RS02730 (P5636_02730) - 494335..494820 (-) 486 WP_013083987.1 hypothetical protein -
  P5636_RS02735 (P5636_02735) fsa 494898..495566 (-) 669 WP_013083988.1 fructose-6-phosphate aldolase -
  P5636_RS02740 (P5636_02740) gnd 495616..496509 (-) 894 WP_013083989.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15356.20 Da        Isoelectric Point: 4.4990

>NTDB_id=806745 P5636_RS02715 WP_013083984.1 492497..492919(+) (nucA/comI) [Priestia megaterium DSM 319]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGNGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=806745 P5636_RS02715 WP_013083984.1 492497..492919(+) (nucA/comI) [Priestia megaterium DSM 319]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGTGCTGAAGAAAACCGCCGTGAATCTTTAAAA
GGCATTCCGACTAAAAGCGGCTACGATCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAACGGTGCAGACAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGATCTTGGATCTCACATCAAGTAGACGAGTTTCCAGACGGCACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q8UP88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507