Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P5650_RS10855 Genome accession   NZ_CP120608
Coordinates   1603681..1604733 (-) Length   350 a.a.
NCBI ID   WP_013085018.1    Uniprot ID   D5DMH8
Organism   Priestia megaterium strain DSM 32     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1598681..1609733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5650_RS10815 (P5650_10805) - 1599697..1600521 (+) 825 WP_016765555.1 YqhG family protein -
  P5650_RS10820 (P5650_10810) - 1600546..1600730 (-) 185 Protein_1724 YqzE family protein -
  P5650_RS10825 (P5650_10815) comGG 1600774..1601148 (-) 375 WP_016765556.1 competence type IV pilus minor pilin ComGG -
  P5650_RS10830 (P5650_10820) comGF 1601138..1601575 (-) 438 WP_034648933.1 competence type IV pilus minor pilin ComGF -
  P5650_RS10835 (P5650_10825) - 1601572..1601910 (-) 339 WP_016765558.1 type II secretion system protein -
  P5650_RS10840 (P5650_10830) comGD 1601894..1602331 (-) 438 WP_016765559.1 competence type IV pilus minor pilin ComGD -
  P5650_RS10845 (P5650_10835) comGC 1602318..1602629 (-) 312 WP_016765560.1 competence type IV pilus major pilin ComGC -
  P5650_RS10850 (P5650_10840) comGB 1602644..1603690 (-) 1047 WP_034648932.1 competence type IV pilus assembly protein ComGB -
  P5650_RS10855 (P5650_10845) comGA 1603681..1604733 (-) 1053 WP_013085018.1 competence type IV pilus ATPase ComGA Machinery gene
  P5650_RS10860 (P5650_10850) - 1605002..1605385 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  P5650_RS10865 (P5650_10855) - 1605648..1605884 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  P5650_RS10870 (P5650_10860) - 1605922..1606554 (-) 633 WP_034648929.1 MBL fold metallo-hydrolase -
  P5650_RS10875 (P5650_10865) - 1606763..1606939 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  P5650_RS10880 (P5650_10870) - 1606975..1607877 (-) 903 WP_016765563.1 M14 family metallocarboxypeptidase -
  P5650_RS10885 (P5650_10875) - 1607989..1608957 (-) 969 WP_016765565.1 ROK family glucokinase -
  P5650_RS10890 (P5650_10880) - 1608960..1609166 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39671.03 Da        Isoelectric Point: 9.1859

>NTDB_id=806699 P5650_RS10855 WP_013085018.1 1603681..1604733(-) (comGA) [Priestia megaterium strain DSM 32]
MTEIEQFATYVIRDAYECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=806699 P5650_RS10855 WP_013085018.1 1603681..1604733(-) (comGA) [Priestia megaterium strain DSM 32]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATATGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGAGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACATTAGAAGATCCTATTGAAAAACCGAGCGAAACCGTCCTTCAAGTACA
GGTAAATGAACGGGCAGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGCGTTAGCTTTCAAGAAATGGAACAGACCCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGAA
AACATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5DMH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.857

100

0.589