Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5650_RS07710 Genome accession   NZ_CP120608
Coordinates   972201..972638 (+) Length   145 a.a.
NCBI ID   WP_034649597.1    Uniprot ID   A0A0B6AKA8
Organism   Priestia megaterium strain DSM 32     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 967201..977638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5650_RS07690 (P5650_07685) - 967757..968428 (-) 672 WP_034649601.1 response regulator transcription factor -
  P5650_RS07695 (P5650_07690) - 968754..969572 (+) 819 WP_013058559.1 phytoene/squalene synthase family protein -
  P5650_RS07700 (P5650_07695) - 969608..971557 (-) 1950 WP_034649599.1 FUSC family protein -
  P5650_RS07705 (P5650_07700) - 971714..971974 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  P5650_RS07710 (P5650_07705) nucA/comI 972201..972638 (+) 438 WP_034649597.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P5650_RS07715 (P5650_07710) comJ 972674..973081 (+) 408 WP_016765176.1 competence protein ComJ -
  P5650_RS07720 (P5650_07715) helD 973110..975428 (-) 2319 WP_034649592.1 RNA polymerase recycling motor HelD -
  P5650_RS07725 (P5650_07720) - 975673..975798 (-) 126 WP_016765178.1 hypothetical protein -
  P5650_RS07730 (P5650_07725) - 975939..976454 (-) 516 WP_034655000.1 enoyl-CoA hydratase/isomerase family protein -
  P5650_RS07735 (P5650_07730) - 976696..977064 (+) 369 WP_013084552.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15933.81 Da        Isoelectric Point: 4.4411

>NTDB_id=806693 P5650_RS07710 WP_034649597.1 972201..972638(+) (nucA/comI) [Priestia megaterium strain DSM 32]
MATYGKKLIASLFIMACILGFMGHGAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANR
EESLKGYPTKIGYDRDEWPMAMCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 438 bp        

>NTDB_id=806693 P5650_RS07710 WP_034649597.1 972201..972638(+) (nucA/comI) [Priestia megaterium strain DSM 32]
ATGGCTACATACGGGAAAAAATTAATAGCAAGTCTATTCATCATGGCATGTATTTTAGGGTTTATGGGACATGGAGCTTT
TTTAGCTCCCGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGACCGATATCCTGAAACGG
GTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACGGTGCTGAAGCGAACCGA
GAAGAATCCCTGAAAGGATATCCGACAAAAATAGGTTATGACCGTGATGAATGGCCAATGGCTATGTGCGAAGAAGGCGG
AGCAGGAGCTGACATTCGCTATATTTCGCCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGGAAATCAGTTAGAACAGT
ATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(51-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B6AKA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

64.286

86.897

0.559