Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   P5661_RS05640 Genome accession   NZ_CP120600
Coordinates   1093807..1097505 (+) Length   1232 a.a.
NCBI ID   WP_277709388.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO112     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1088807..1102505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5661_RS05625 (P5661_05625) yhjQ 1089255..1089581 (-) 327 WP_009966950.1 four-helix bundle copper-binding protein -
  P5661_RS05630 (P5661_05630) yhjR 1089699..1090136 (-) 438 WP_003245806.1 ferritin family protein -
  P5661_RS05635 (P5661_05635) addB 1090320..1093820 (+) 3501 WP_277709387.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  P5661_RS05640 (P5661_05640) addA 1093807..1097505 (+) 3699 WP_277709388.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  P5661_RS05645 (P5661_05645) sbcD 1097577..1098752 (+) 1176 WP_032721297.1 exonuclease subunit SbcD -
  P5661_RS05650 (P5661_05650) sbcC 1098749..1102141 (+) 3393 WP_277709389.1 exonuclease subunit SbcC -
  P5661_RS05655 (P5661_05655) hlpB 1102155..1102457 (+) 303 WP_015252406.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 140920.10 Da        Isoelectric Point: 5.0405

>NTDB_id=806557 P5661_RS05640 WP_277709388.1 1093807..1097505(+) (addA) [Bacillus subtilis strain PRO112]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDEA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELSFYQYVKEDIAMVLNGAREKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFNELYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIDTLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGERGPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGIGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNGETETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFQVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVAISVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNGELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
LGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEDLAGVPAHADISDHPARFAVQMIHSHDLL
DDDLEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPADG
SILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAIDIEEIVQFFHTEIGGQL
IGAKWKDREIPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYETQ
IQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=806557 P5661_RS05640 WP_277709388.1 1093807..1097505(+) (addA) [Bacillus subtilis strain PRO112]
ATGAACATTCCTAAACCGGCAGACAGCACGTGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CAATAGATGTAGACCGTCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAGCACCGAATCGCAGAAGCC
TTGGAAAAAGAGCTTGTACAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTATTTGATCGATCTTGATCCGGGCTTTCGGATTGCCG
ATCAGACCGAAGGCGAGCTGCTTGGGGATGAAGTGCTGGATGAGCTGTTCGAAGACGAATACGCAAAGGGTGACGAGGCG
TTTTTTGAGCTGGTTGACCGCTACACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGATCCCATCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCTGAAAAGAGCGCGATCG
AAGAGCTTTCGTTTTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGAGCGAGGGAAAAGCTCCTGCGCGCC
CTTGAACTGACAAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGACAATTTCCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCAATGAACTTTATAAGCGGGTGCCTGCCGTCTCATTTAAGCGCGCGAAGGCGGTAAAAG
GGGACGAGTTTGATCCCGCGCTGCTTGATGAAGCGACAGACTTGCGAAATGGCGCGAAAAAACTGCTTGAAAAGCTCAAA
ACCGACTACTTCACAAGAAGTCCTGAACAGCATTTGAAAAGCCTAGCCGAGATGAAGCCTGTCATTGATACACTCGTACA
GCTTGTCATTAGCTATGGAAAGCGATTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTGGCGATTTTGACAGCTGAAAATGACAAAGGCGAACGCGGTCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTGCAGGAATCGATCCTGCAGCTTGTCACAAGCGGGCC
GGAGGAGACTGGAAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAATAGGAACGGGACGGAAGATCGACTTGAATAAAAACTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTGGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGCGCGGCGTATCCGGACAATGGCGAGACGGAAACAGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAACTGATTTCATCACCGTTTCAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTCGCCGTCGCGATTTCGGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAACACAAA
AGCTCCGTACTATGAAGCGATGAAAGACTACTTGGCAGCCGGCGACCGGAACGGCGAGCTTTATCAAAAGTTGCAAACCT
TTTATGGGCATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTCCTTTACGACCGGGC
GCGCCAATATGAATCAACGGCGTTTCGCGGCCTGTTCCGTTTCCTGCGATTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAGGGGCTTGAA
TTCCCTGTTGTGTTTGCAGCGGGTCTCGGCCGCAATTTCAACATGATGGACTTAAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTATATTCACCCGCAATTGCGTATCAGCTATCCGACGCTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGGGTACTCTATGTTGCATTAACAAGAGCAAAGGAAAAATTGTTTCTG
CTTGGCTCGTGCAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCATCTGCGTCACAAACTGATTGGCTTCTTCCGGA
GTTTGACCGCTATCAGGCAAAAACGTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGACTTGGAGGATTTGG
CTGGTGTGCCAGCACACGCTGACATCTCAGATCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCCATGATTTGCTT
GATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCCCGGCTC
GTTTGCGTTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCGCATCACGAGGTGACGCAAATTCGGACAAAGC
AATCGGTTTCTGAGATCAAAAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTAAAGCCGGCTGATGGA
AGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGGACTGCCATGCATACGGT
TATGCAGCATATCCCGCTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCACAGGCTTTATGAAAAAGAGC
TTCTCACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGACAGCTG
ATCGGCGCAAAGTGGAAGGACAGGGAAATACCATTCAGCTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGCACAGGA
GGCAGATGAGCCGCTTTTAGTGCAGGGTATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATCTATTGGATTATA
AGTCGGACCGGATTGAGGGCAAATTCCAGCATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAAACGCAA
ATTCAGCTGTACACGAAGGCAGTGGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGACGGAGG
GCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-880)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1053-1223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

97.808

100

0.978