Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P5638_RS02415 Genome accession   NZ_CP120599
Coordinates   493321..494700 (-) Length   459 a.a.
NCBI ID   WP_024425580.1    Uniprot ID   -
Organism   Bacillus safensis strain PRO114     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 488321..499700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5638_RS02395 (P5638_02395) ispF 489802..490278 (-) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  P5638_RS02400 (P5638_02400) ispD 490282..490971 (-) 690 WP_024425583.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P5638_RS02405 (P5638_02405) - 490985..492085 (-) 1101 WP_024425582.1 PIN/TRAM domain-containing protein -
  P5638_RS02410 (P5638_02410) disA 492239..493318 (-) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  P5638_RS02415 (P5638_02415) radA 493321..494700 (-) 1380 WP_024425580.1 DNA repair protein RadA Machinery gene
  P5638_RS02420 (P5638_02420) clpC 494794..497229 (-) 2436 WP_007496263.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  P5638_RS02425 (P5638_02425) - 497226..498317 (-) 1092 WP_024425579.1 protein arginine kinase -
  P5638_RS02430 (P5638_02430) - 498322..498879 (-) 558 WP_024425862.1 UvrB/UvrC motif-containing protein -
  P5638_RS02435 (P5638_02435) - 498894..499358 (-) 465 WP_003217184.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50039.51 Da        Isoelectric Point: 7.3829

>NTDB_id=806482 P5638_RS02415 WP_024425580.1 493321..494700(-) (radA) [Bacillus safensis strain PRO114]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=806482 P5638_RS02415 WP_024425580.1 493321..494700(-) (radA) [Bacillus safensis strain PRO114]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACAAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCTCTTGTTCTCATCGGCGGAGATCCTGGGATTGGGAAGTCCACATT
GTTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCGTTTGTTGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
TCCCGGCTCTGTATCTCAAGTTAGAGAATGTACAGCGCAGCTGATGAAGATTGCCAAAACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATTTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTCGAGATACAGGCGTTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGACCATAATCGAGTATCGCTGCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCCTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCTA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCAAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGACGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608