Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5638_RS01085 Genome accession   NZ_CP120599
Coordinates   244068..244496 (+) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain PRO114     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 239068..249496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5638_RS01075 (P5638_01075) - 239653..241641 (-) 1989 WP_125587105.1 hydantoinase B/oxoprolinase family protein -
  P5638_RS01080 (P5638_01080) - 241631..243700 (-) 2070 WP_024427271.1 hydantoinase/oxoprolinase family protein -
  P5638_RS01085 (P5638_01085) nucA/comI 244068..244496 (+) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P5638_RS01090 (P5638_01090) - 244624..246321 (+) 1698 WP_034624006.1 methyl-accepting chemotaxis protein -
  P5638_RS01095 (P5638_01095) comJ 246546..246827 (+) 282 WP_277722962.1 competence protein ComJ -
  P5638_RS01100 (P5638_01100) - 246829..247635 (-) 807 WP_025092812.1 AAC(3) family N-acetyltransferase -
  P5638_RS01105 (P5638_01105) - 247664..248080 (-) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  P5638_RS01110 (P5638_01110) - 248196..248960 (+) 765 WP_034624002.1 MBL fold metallo-hydrolase -
  P5638_RS01115 (P5638_01115) - 249006..249335 (-) 330 WP_024424311.1 YckD family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=806481 P5638_RS01085 WP_024424305.1 244068..244496(+) (nucA/comI) [Bacillus safensis strain PRO114]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=806481 P5638_RS01085 WP_024424305.1 244068..244496(+) (nucA/comI) [Bacillus safensis strain PRO114]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTACCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535