Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   P5622_RS19035 Genome accession   NZ_CP120598
Coordinates   3637253..3640951 (-) Length   1232 a.a.
NCBI ID   WP_003233100.1    Uniprot ID   A0AAE2SH43
Organism   Bacillus subtilis strain PRO115     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3632253..3645951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5622_RS19020 (P5622_19020) hlpB 3632301..3632603 (-) 303 WP_003245113.1 HNH nuclease-like protein HlpB -
  P5622_RS19025 (P5622_19025) sbcC 3632617..3636009 (-) 3393 WP_003245201.1 exonuclease subunit SbcC -
  P5622_RS19030 (P5622_19030) sbcD 3636006..3637181 (-) 1176 WP_003233099.1 exonuclease subunit SbcD -
  P5622_RS19035 (P5622_19035) addA 3637253..3640951 (-) 3699 WP_003233100.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  P5622_RS19040 (P5622_19040) addB 3640938..3644438 (-) 3501 WP_003244988.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  P5622_RS19045 (P5622_19045) yhjR 3644622..3645059 (+) 438 WP_003245806.1 ferritin family protein -
  P5622_RS19050 (P5622_19050) yhjQ 3645177..3645503 (+) 327 WP_009966950.1 four-helix bundle copper-binding protein -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 141087.44 Da        Isoelectric Point: 5.0963

>NTDB_id=806468 P5622_RS19035 WP_003233100.1 3637253..3640951(-) (addA) [Bacillus subtilis strain PRO115]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELIGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKKKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRSDELYQKLNTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFVAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQARTYLDFIGPALARHRDLGDLAGVPAHADISGHPARFAVQMIHSYDLL
DDDLEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPADG
SILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAIDIEEIVQFFHTEIGGQL
IGAKWKDREIPFSLALPAKEIYPDAHEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYETQ
IQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=806468 P5622_RS19035 WP_003233100.1 3637253..3640951(-) (addA) [Bacillus subtilis strain PRO115]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CAATAGATGTAGACCGTCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAGCACCGAATCGCAGAAGCC
TTGGAAAAAGAGCTTGTACAGCGCCCCGGCTCGCTGCATATTAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACGCTCCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGATCGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTATACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTTAAACAGGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCAGCGCTCCTTGATGAGGCGACAGATTTGAGGAACGGCGCAAAAAAACTGCTTGAAAAGCTCAAA
ACCGACTACTTCACGCGAAGTCCTGAACAGCACTTGAAAAGCCTAGCCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGATTCGAAGCTGCGAAACAGGAAAAATCAATCATCGATTTTTCGGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTGAGAATGACAAAGGTGAACGTGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAACAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTGCAGGAATCGATTCTGCAGCTCGTCACAAGCGGTCC
GGAGGAGACTGGTAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGGCGGAAAATCGATTTAAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCAGCGTATCCGGACAATGACGAGACGGAAACAGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACGGTGCAGTTTGAGGCAAAAGCCATCGCTAAGGAAATTCGT
AAGCTGATTTCATCGCCGTTTAAGGTGTATGACGGAAAAAAGAAAACACATCGCAATATTCAATACCGAGATATCGTGAT
TTTGCTCCGTTCGATGCCGTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATTGATAATCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAGGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAGCGATGAGCTTTATCAAAAGTTAAATACGT
TTTACGGACATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACCAAATATATGGATTATGTCGGCGGCATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGTCAATATGAATCAACGGCATTTCGCGGCTTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGA
GCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGACACTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTGCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCATGTAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAGAACGTATCTAGATTTCATTGGGCCGGCTCTTGCCAGGCACAGAGACTTGGGGGATTTGG
CTGGTGTGCCAGCACACGCTGACATCTCAGGTCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCTATGATTTGCTT
GATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCCCGGCTC
GTTTGCGTTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACCTACCCGCATCAAGAAGTGACGCAAATTCGGACAAAGC
AATCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTAAAACCGGCTGATGGA
AGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGGACTGCCATGCATACGGT
TATGCAGCATATCCCGCTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCACAGGCTTTATGAAAAAGAGC
TTCTCACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGACAGCTG
ATCGGTGCTAAGTGGAAGGACCGGGAAATACCATTCAGCTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGCACACGA
GGCAGATGAGCCGCTTTTAGTGCAGGGTATTATTGACTGTCTCTATGAAACTGAGGACGGATTATATCTATTGGATTATA
AGTCGGACCGGATTGAGGGCAAATTCCAGCATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAAACGCAA
ATTCAGCTGTACACGAAGGCAGTGGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGACGGAGG
GCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1053-1223)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

100

100

1