Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5622_RS01270 Genome accession   NZ_CP120598
Coordinates   247116..247565 (+) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis strain PRO115     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 242116..252565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5622_RS01250 (P5622_01250) hxlR 243327..243689 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  P5622_RS01255 (P5622_01255) hxlA 243921..244553 (+) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  P5622_RS01260 (P5622_01260) hxlB 244559..245116 (+) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  P5622_RS01265 (P5622_01265) tlpC 245227..246948 (+) 1722 WP_277687281.1 methyl-accepting chemotaxis protein TlpC -
  P5622_RS01270 (P5622_01270) nucA/comI 247116..247565 (+) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  P5622_RS01275 (P5622_01275) nin/comJ 247592..247990 (+) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  P5622_RS01280 (P5622_01280) bglC 248027..249460 (-) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  P5622_RS01285 (P5622_01285) yckD 249614..249946 (-) 333 WP_003246247.1 YckD family protein -
  P5622_RS01290 (P5622_01290) yckC 250028..250483 (-) 456 WP_003246356.1 RDD family protein -
  P5622_RS01295 (P5622_01295) - 250502..250699 (-) 198 WP_119122841.1 hypothetical protein -
  P5622_RS01300 (P5622_01300) yckB 250861..251724 (+) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  P5622_RS01305 (P5622_01305) yckA 251734..252414 (+) 681 WP_003234624.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=806406 P5622_RS01270 WP_003246436.1 247116..247565(+) (nucA/comI) [Bacillus subtilis strain PRO115]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=806406 P5622_RS01270 WP_003246436.1 247116..247565(+) (nucA/comI) [Bacillus subtilis strain PRO115]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1