Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P5663_RS06820 Genome accession   NZ_CP120590
Coordinates   1309936..1310994 (+) Length   352 a.a.
NCBI ID   WP_025911559.1    Uniprot ID   -
Organism   Priestia flexa strain PRO116     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1304936..1315994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5663_RS06795 (P5663_06795) - 1306804..1307712 (+) 909 WP_277717338.1 M14 family metallopeptidase -
  P5663_RS06800 (P5663_06800) - 1307769..1307942 (-) 174 WP_025911564.1 DUF2759 domain-containing protein -
  P5663_RS06805 (P5663_06805) - 1308139..1308771 (+) 633 WP_025911563.1 MBL fold metallo-hydrolase -
  P5663_RS06810 (P5663_06810) - 1308815..1309054 (-) 240 WP_025911561.1 DUF2626 domain-containing protein -
  P5663_RS06815 (P5663_06815) - 1309334..1309717 (+) 384 WP_061784685.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  P5663_RS06820 (P5663_06820) comGA 1309936..1310994 (+) 1059 WP_025911559.1 competence type IV pilus ATPase ComGA Machinery gene
  P5663_RS06825 (P5663_06825) comGB 1310975..1312033 (+) 1059 WP_025911558.1 competence type IV pilus assembly protein ComGB -
  P5663_RS06830 (P5663_06830) - 1312047..1312205 (+) 159 Protein_1258 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P5663_RS06835 (P5663_06835) - 1312440..1313201 (+) 762 WP_277717339.1 hypothetical protein -
  P5663_RS06840 (P5663_06840) - 1313304..1313624 (+) 321 WP_277717340.1 hypothetical protein -
  P5663_RS06845 (P5663_06845) - 1313644..1313874 (-) 231 WP_277717341.1 helix-turn-helix domain-containing protein -
  P5663_RS06850 (P5663_06850) - 1314077..1314499 (+) 423 WP_277717342.1 hypothetical protein -
  P5663_RS06855 (P5663_06855) - 1314643..1314864 (+) 222 WP_277717343.1 hypothetical protein -
  P5663_RS06860 (P5663_06860) - 1314979..1315221 (+) 243 WP_277717344.1 hypothetical protein -
  P5663_RS06865 (P5663_06865) - 1315299..1315544 (+) 246 WP_277717345.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39853.99 Da        Isoelectric Point: 8.3459

>NTDB_id=806374 P5663_RS06820 WP_025911559.1 1309936..1310994(+) (comGA) [Priestia flexa strain PRO116]
MNEIEQLATRMIREACEMKSSDIHIIPRARDAIIQLRVDQDLVAHQTLPKEVSERLISHFKFLGSMDIGERRKPQNGALS
TTIDHIKVNLRLSTLPTLQDESLVIRVLPQTQTTSLKYLSLFQNHTKKLISLLKHSHGLIVFTGPTGSGKTTTLYTLLHE
VNHLLNRNVITLEDPIEQQSEKVLQVQVNERAGITYATGLKAILRHDPDIIMVGEIRDAETAQIAVRAALTGHLVLTTMH
TKDTKGSIYRLLEFGVSIQEIEQTLVAVAAQRLVEIKCNFCEGRCHPLCKKERLNRQASVYELLYGREVNEVINEVKGKE
FQYDYARLRDAIRKGIALGYLYSHALDGWGSY

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=806374 P5663_RS06820 WP_025911559.1 1309936..1310994(+) (comGA) [Priestia flexa strain PRO116]
TTGAATGAAATTGAACAATTAGCCACACGCATGATTCGTGAAGCATGTGAGATGAAGAGCTCGGATATTCACATTATCCC
TCGAGCTAGAGATGCCATAATTCAGCTAAGAGTTGATCAAGATTTGGTTGCTCATCAAACGCTCCCAAAAGAGGTATCTG
AACGACTCATTTCCCACTTTAAATTTTTAGGGAGCATGGATATTGGTGAAAGACGAAAGCCTCAAAATGGTGCACTGTCT
ACAACAATCGATCATATTAAAGTTAATCTACGTTTATCCACTTTACCTACATTGCAGGATGAAAGTCTCGTTATTCGTGT
ATTACCCCAAACGCAAACAACTTCTCTTAAATATTTATCACTTTTTCAAAATCATACCAAAAAACTTATTTCACTGCTTA
AGCATTCACATGGATTAATTGTGTTTACTGGGCCAACAGGATCCGGTAAAACAACAACTTTGTATACGTTACTTCATGAG
GTCAACCATTTATTAAATCGAAATGTCATTACGCTTGAAGATCCAATTGAACAGCAGAGTGAAAAAGTACTGCAGGTACA
AGTGAATGAACGAGCTGGCATCACGTATGCAACAGGTTTAAAAGCAATTTTACGTCATGACCCTGATATTATTATGGTCG
GGGAAATCCGCGACGCTGAAACAGCTCAAATTGCGGTTCGCGCAGCTTTAACCGGTCATTTAGTACTGACAACTATGCAT
ACGAAAGATACAAAAGGCTCTATTTATCGATTGCTTGAGTTTGGTGTTAGTATTCAAGAAATTGAACAAACGTTGGTAGC
CGTAGCTGCACAGCGATTAGTCGAAATAAAATGTAATTTTTGTGAAGGAAGATGTCATCCGCTATGTAAGAAAGAGCGTT
TAAATAGACAGGCTAGCGTGTATGAGCTTCTATATGGAAGGGAAGTAAACGAAGTAATTAATGAAGTGAAAGGAAAGGAA
TTTCAATATGATTATGCACGACTAAGAGATGCAATTCGAAAAGGTATTGCGCTTGGGTATTTGTATTCACATGCGCTAGA
TGGGTGGGGGTCTTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

60

99.432

0.597