Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P5663_RS02205 Genome accession   NZ_CP120590
Coordinates   416423..417802 (-) Length   459 a.a.
NCBI ID   WP_277717139.1    Uniprot ID   -
Organism   Priestia flexa strain PRO116     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 411423..422802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5663_RS02185 (P5663_02185) ispF 412894..413373 (-) 480 WP_025909703.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  P5663_RS02190 (P5663_02190) ispD 413386..414075 (-) 690 WP_277717138.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P5663_RS02195 (P5663_02195) - 414101..415198 (-) 1098 WP_061784782.1 PIN/TRAM domain-containing protein -
  P5663_RS02200 (P5663_02200) disA 415341..416417 (-) 1077 WP_078988977.1 DNA integrity scanning diadenylate cyclase DisA -
  P5663_RS02205 (P5663_02205) radA 416423..417802 (-) 1380 WP_277717139.1 DNA repair protein RadA Machinery gene
  P5663_RS02210 (P5663_02210) clpC 417926..420367 (-) 2442 WP_277717140.1 ATP-dependent protease ATP-binding subunit ClpC -
  P5663_RS02215 (P5663_02215) - 420388..421455 (-) 1068 WP_025909697.1 protein arginine kinase -
  P5663_RS02220 (P5663_02220) - 421460..421999 (-) 540 WP_025909696.1 UvrB/UvrC motif-containing protein -
  P5663_RS02225 (P5663_02225) - 422037..422498 (-) 462 WP_078988979.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49723.97 Da        Isoelectric Point: 7.4549

>NTDB_id=806349 P5663_RS02205 WP_277717139.1 416423..417802(-) (radA) [Priestia flexa strain PRO116]
MSKRKTKFSCQHCGYESAKWMGKCPGCGSWNSMVEEIEETKSSRRGGAFSGATSTKVQKPQSITNIESTTEPRIYTKSPE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMNKHKVLYISGEESTKQTKLRADRLGIKAEDLYVHAETNLESI
TEAISSMNPELVIIDSIQTIYHGDVTSAPGSVSQVRECTAELMRIAKTNGIAIFIVGHVTKEGSIAGPRLLEHMVDSVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTS
FGNPRRMSTGLDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFKDAPSNPTDVVVGEVGL
TGEIRRVSRIEQRVQEAVKLGFKRIIVPEKNLGGWKIPEGIDVVGVSTVSEALQYTLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=806349 P5663_RS02205 WP_277717139.1 416423..417802(-) (radA) [Priestia flexa strain PRO116]
ATGAGTAAAAGAAAAACAAAGTTTTCATGTCAACATTGTGGGTATGAATCGGCTAAATGGATGGGAAAGTGTCCTGGCTG
TGGCAGTTGGAATTCAATGGTGGAAGAAATTGAAGAGACAAAGTCTTCAAGGCGAGGAGGAGCTTTTTCTGGCGCAACGT
CTACAAAGGTGCAAAAGCCTCAATCCATTACGAATATTGAATCAACGACTGAACCAAGGATATATACAAAGTCACCCGAG
CTAAACCGTGTTTTAGGTGGAGGGATTGTTAGAGGATCACTAGTCTTAATTGGGGGAGATCCTGGTATTGGTAAATCAAC
TTTGTTGCTACAAACTTCAGCTCAGTTAGCTATGAACAAGCATAAGGTGCTTTATATATCTGGTGAGGAATCAACAAAGC
AAACAAAGCTAAGAGCAGATCGCTTAGGTATTAAAGCGGAAGATTTGTATGTGCATGCAGAAACAAACTTAGAGTCAATC
ACAGAAGCTATCTCAAGTATGAATCCAGAACTTGTTATCATTGATTCAATTCAAACAATTTATCACGGTGACGTTACATC
AGCGCCCGGAAGTGTTTCTCAAGTACGCGAGTGTACAGCAGAGTTAATGCGAATTGCAAAAACAAACGGCATAGCTATTT
TTATTGTAGGTCATGTTACCAAAGAAGGGTCTATTGCAGGTCCAAGGTTACTTGAACATATGGTAGACAGTGTTTTGTAT
TTTGAAGGAGAACGCCACCATACGTACCGGATTTTAAGAGCAGTAAAAAACCGCTTTGGTTCAACAAACGAAATGGGTAT
TTTTGAAATGAAGGAAAGTGGTCTAGAGGAAGTGCTCAACCCATCTGAAATCTTTTTAGAAGAACGATCTCAAGGTGCAG
CTGGTTCTGTCGTTGTGGCTTCGATGGAGGGTACTCGTCCCGTATTGGTAGAGTTACAAGCACTTATTAGCCCAACAAGC
TTTGGAAATCCTAGAAGAATGTCTACAGGACTTGATCATAACCGTGTCTCGCTTATTATGGCAGTGTTAGAAAAGCGAGT
AGGATTACTGTTGCAAAATCAAGATGCGTACTTAAAAGTAGCAGGTGGGGTAAAGTTAGATGAGCCAGCTATTGATTTGG
CGGTTGCGGTTAGCATTGCATCGAGTTTTAAAGATGCACCTTCAAACCCTACAGACGTAGTAGTAGGAGAAGTTGGATTA
ACAGGTGAGATTCGAAGAGTGTCTCGCATTGAACAACGAGTGCAAGAAGCTGTGAAGTTAGGATTTAAGCGAATCATTGT
TCCTGAAAAAAACTTAGGTGGTTGGAAAATACCAGAAGGCATTGACGTAGTAGGGGTGTCCACTGTGTCAGAAGCGTTAC
AGTATACGTTAGGAGGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.163

100

0.752
  radA Streptococcus pneumoniae Rx1

63.436

98.911

0.627
  radA Streptococcus pneumoniae D39

63.436

98.911

0.627
  radA Streptococcus pneumoniae R6

63.436

98.911

0.627
  radA Streptococcus pneumoniae TIGR4

63.436

98.911

0.627
  radA Streptococcus mitis NCTC 12261

63.436

98.911

0.627
  radA Streptococcus mitis SK321

63.436

98.911

0.627