Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   P5628_RS12655 Genome accession   NZ_CP120577
Coordinates   2435766..2436533 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain PRO53     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2430766..2441533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS12635 (P5628_12635) ppsR 2430940..2431752 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  P5628_RS12640 (P5628_12640) ccpN 2431778..2432416 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  P5628_RS12645 (P5628_12645) glyS 2432549..2434588 (-) 2040 WP_273797497.1 glycine--tRNA ligase subunit beta -
  P5628_RS12650 (P5628_12650) glyQ 2434581..2435468 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  P5628_RS12655 (P5628_12655) recO 2435766..2436533 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  P5628_RS12660 (P5628_12660) yqzL 2436570..2436713 (-) 144 WP_003230053.1 YqzL family protein -
  P5628_RS12665 (P5628_12665) era 2436861..2437766 (-) 906 WP_003230051.1 GTPase Era -
  P5628_RS12670 (P5628_12670) cdd 2437747..2438157 (-) 411 WP_003230049.1 cytidine deaminase -
  P5628_RS12675 (P5628_12675) unpK 2438276..2438647 (-) 372 WP_277708800.1 diacylglycerol kinase family protein -
  P5628_RS12680 (P5628_12680) ybeY 2438628..2439101 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  P5628_RS12685 (P5628_12685) pgpH 2439102..2441237 (-) 2136 WP_277708801.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=806314 P5628_RS12655 WP_014477369.1 2435766..2436533(-) (recO) [Bacillus subtilis strain PRO53]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=806314 P5628_RS12655 WP_014477369.1 2435766..2436533(-) (recO) [Bacillus subtilis strain PRO53]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGTACTGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTTCATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGTATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996