Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5628_RS01865 Genome accession   NZ_CP120577
Coordinates   348239..348688 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain PRO53     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 343239..353688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS01825 (P5628_01825) - 343560..343757 (+) 198 WP_122060560.1 hypothetical protein -
  P5628_RS01830 (P5628_01830) yckC 343777..344232 (+) 456 WP_049140212.1 RDD family protein -
  P5628_RS01835 (P5628_01835) yckD 344313..344645 (+) 333 WP_032724908.1 YckD family protein -
  P5628_RS01840 (P5628_01840) - 344773..345195 (+) 423 WP_170939093.1 MarR family transcriptional regulator -
  P5628_RS01845 (P5628_01845) - 345183..345524 (+) 342 WP_032724911.1 DUF3147 family protein -
  P5628_RS01850 (P5628_01850) - 345543..346271 (+) 729 WP_021481826.1 AAA family ATPase -
  P5628_RS01855 (P5628_01855) bglC 346344..347777 (+) 1434 WP_038828490.1 6-phospho-beta-glucosidase -
  P5628_RS01860 (P5628_01860) nin/comJ 347814..348212 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  P5628_RS01865 (P5628_01865) nucA/comI 348239..348688 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  P5628_RS01870 (P5628_01870) tlpC 348856..350577 (-) 1722 WP_021481821.1 methyl-accepting chemotaxis protein TlpC -
  P5628_RS01875 (P5628_01875) hxlB 350687..351244 (-) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  P5628_RS01880 (P5628_01880) hxlA 351250..351882 (-) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  P5628_RS01885 (P5628_01885) hxlR 352114..352476 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=806272 P5628_RS01865 WP_014475770.1 348239..348688(-) (nucA/comI) [Bacillus subtilis strain PRO53]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=806272 P5628_RS01865 WP_014475770.1 348239..348688(-) (nucA/comI) [Bacillus subtilis strain PRO53]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987