Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P5628_RS00430 Genome accession   NZ_CP120577
Coordinates   75907..77283 (+) Length   458 a.a.
NCBI ID   WP_014475593.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO53     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 70907..82283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS00410 (P5628_00410) ctsR 71260..71724 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  P5628_RS00415 (P5628_00415) mcsA 71738..72295 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  P5628_RS00420 (P5628_00420) mcsB 72295..73386 (+) 1092 WP_003235007.1 protein arginine kinase -
  P5628_RS00425 (P5628_00425) clpC 73383..75815 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  P5628_RS00430 (P5628_00430) radA 75907..77283 (+) 1377 WP_014475593.1 DNA repair protein RadA Machinery gene
  P5628_RS00435 (P5628_00435) disA 77287..78369 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  P5628_RS00440 (P5628_00440) yacL 78485..79585 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  P5628_RS00445 (P5628_00445) ispD 79600..80298 (+) 699 WP_041850244.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P5628_RS00450 (P5628_00450) ispF 80291..80767 (+) 477 WP_038827792.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49466.99 Da        Isoelectric Point: 8.2399

>NTDB_id=806269 P5628_RS00430 WP_014475593.1 75907..77283(+) (radA) [Bacillus subtilis strain PRO53]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=806269 P5628_RS00430 WP_014475593.1 75907..77283(+) (radA) [Bacillus subtilis strain PRO53]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGA
TTGTGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTAAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.782

100

0.998
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

62.914

98.908

0.622