Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P3L57_RS12920 Genome accession   NZ_CP120511
Coordinates   2603943..2604368 (-) Length   141 a.a.
NCBI ID   WP_044154477.1    Uniprot ID   -
Organism   Bacillus sp. B28     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2598943..2609368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3L57_RS12890 (P3L57_12890) - 2599183..2599317 (-) 135 WP_010335019.1 hypothetical protein -
  P3L57_RS12895 (P3L57_12895) - 2599395..2600126 (-) 732 WP_044154481.1 SGNH/GDSL hydrolase family protein -
  P3L57_RS12900 (P3L57_12900) - 2600373..2601125 (-) 753 WP_069487172.1 N-acetylmuramoyl-L-alanine amidase -
  P3L57_RS12905 (P3L57_12905) - 2601320..2601946 (+) 627 WP_059335451.1 TVP38/TMEM64 family protein -
  P3L57_RS12910 (P3L57_12910) gnd 2601967..2602860 (-) 894 WP_059335452.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  P3L57_RS12915 (P3L57_12915) - 2603181..2603909 (+) 729 WP_044154478.1 hypothetical protein -
  P3L57_RS12920 (P3L57_12920) nucA/comI 2603943..2604368 (-) 426 WP_044154477.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P3L57_RS12925 (P3L57_12925) sigK 2604545..2605273 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  P3L57_RS12930 (P3L57_12930) - 2605459..2605557 (-) 99 Protein_2500 recombinase -
  P3L57_RS12935 (P3L57_12935) - 2605913..2607727 (-) 1815 WP_069487171.1 AAA family ATPase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15556.74 Da        Isoelectric Point: 5.2215

>NTDB_id=805940 P3L57_RS12920 WP_044154477.1 2603943..2604368(-) (nucA/comI) [Bacillus sp. B28]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=805940 P3L57_RS12920 WP_044154477.1 2603943..2604368(-) (nucA/comI) [Bacillus sp. B28]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.29

87.943

0.539