Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   P3L57_RS05720 Genome accession   NZ_CP120511
Coordinates   1169454..1173158 (+) Length   1234 a.a.
NCBI ID   WP_069486349.1    Uniprot ID   -
Organism   Bacillus sp. B28     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1164454..1178158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3L57_RS05705 (P3L57_05705) - 1164902..1165216 (-) 315 WP_044156484.1 four-helix bundle copper-binding protein -
  P3L57_RS05710 (P3L57_05710) - 1165345..1165782 (-) 438 WP_044156482.1 ferritin family protein -
  P3L57_RS05715 (P3L57_05715) addB 1165967..1169467 (+) 3501 WP_069486348.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  P3L57_RS05720 (P3L57_05720) addA 1169454..1173158 (+) 3705 WP_069486349.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  P3L57_RS05725 (P3L57_05725) sbcD 1173230..1174408 (+) 1179 WP_044156478.1 exonuclease subunit SbcD -
  P3L57_RS05730 (P3L57_05730) sbcC 1174405..1177797 (+) 3393 WP_069486350.1 exonuclease subunit SbcC -
  P3L57_RS05735 (P3L57_05735) - 1177811..1178113 (+) 303 WP_044156476.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141462.93 Da        Isoelectric Point: 5.1008

>NTDB_id=805906 P3L57_RS05720 WP_069486349.1 1169454..1173158(+) (addA) [Bacillus sp. B28]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLADMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENSETETELLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKALERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGAKWKNREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=805906 P3L57_RS05720 WP_069486349.1 1169454..1173158(+) (addA) [Bacillus sp. B28]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGATTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCAGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTGAAACGCCCAGGATCGCTGCATATCCGACGCCAGCTGTCGCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTATCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGTTACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCCCGTTCCCACCCTGATCCGGAGACATGGCTGAAAAGTTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTCCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGATCCCGTCTTGCTTGATGAAGCGACAGACTTACGAAATGGCGCCAAAAAACTGCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGACATGGAGCCTGTCATTGAAACACTCGTACA
GCTTGTTATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATCGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTTGTCGATGAATACCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAATCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTCTCCAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACGGGCCGGAAAATCGATTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAGCTGAAACTCGGAGCGGCGTATCCGGAAAACAGCGAAACGGAAACAGAGCTGCTGTTGATTGACAAGTCAG
AGGAGGCGGACGCAGGCGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAGGAACTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAGCTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTCTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGCTTTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGCTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ATCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAGGATGTTGTCCGTTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTCCCAGTCGTTTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCTGAGGAATTGCGTGTCCTCTATGTGGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCCGCGTCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGCGCGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCTCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCCGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGGCGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATTGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGGGAA
AAACTGATCGGCGCTAAATGGAAGAACCGCGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.436

100

0.934