Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P2869_RS01460 Genome accession   NZ_CP120508
Coordinates   319146..320654 (-) Length   502 a.a.
NCBI ID   WP_126877715.1    Uniprot ID   -
Organism   Paraburkholderia sp. SUR17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314146..325654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P2869_RS01435 (P2869_01435) - 314995..315630 (-) 636 WP_277597252.1 LON peptidase substrate-binding domain-containing protein -
  P2869_RS01440 (P2869_01440) rapZ 315740..316633 (-) 894 WP_277597253.1 RNase adapter RapZ -
  P2869_RS01445 (P2869_01445) hprK 316700..317668 (-) 969 WP_017772650.1 HPr(Ser) kinase/phosphatase -
  P2869_RS01450 (P2869_01450) ptsN 317821..318336 (-) 516 WP_017772651.1 PTS IIA-like nitrogen regulatory protein PtsN -
  P2869_RS01455 (P2869_01455) raiA 318615..318974 (-) 360 WP_017772652.1 ribosome-associated translation inhibitor RaiA -
  P2869_RS01460 (P2869_01460) rpoN/rpoN1 319146..320654 (-) 1509 WP_126877715.1 RNA polymerase factor sigma-54 Machinery gene
  P2869_RS01465 (P2869_01465) lptB 320945..321727 (-) 783 WP_017772654.1 LPS export ABC transporter ATP-binding protein -
  P2869_RS01470 (P2869_01470) lptA 321724..322401 (-) 678 WP_017772655.1 lipopolysaccharide transport periplasmic protein LptA -
  P2869_RS01475 (P2869_01475) lptC 322434..323033 (-) 600 WP_199541741.1 LPS export ABC transporter periplasmic protein LptC -
  P2869_RS01480 (P2869_01480) - 323030..323578 (-) 549 WP_277597254.1 HAD family hydrolase -
  P2869_RS01485 (P2869_01485) kdsD 323578..324561 (-) 984 WP_017772658.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54980.09 Da        Isoelectric Point: 5.0002

>NTDB_id=805821 P2869_RS01460 WP_126877715.1 319146..320654(-) (rpoN/rpoN1) [Paraburkholderia sp. SUR17]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENEDDWIASPLRVAADGSVIAQTPTPSAPEPMS
AAPSSSSSTESSESGEPASVDEYNGLGSDANGDASQWNLDDYGRSGNASDDDDLPPLQIHESTTSLRDYLTAQLRVTQAS
PRDRALITFLIESLDDEGYLTATCEEILADLPVELEVDADELNAALALLQSFDPPGVGARSASECLKLQLLRLDPSPTRT
LALEIVAHHLELLAARDFTRLRKHLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDVIVKKTAQGWHAELNPEVV
PKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFVHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=805821 P2869_RS01460 WP_126877715.1 319146..320654(-) (rpoN/rpoN1) [Paraburkholderia sp. SUR17]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCATCTTGCGCTGACCCCCCAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTCGAATTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCCCTGCTCGAAAACGAAGACGACTGGA
TCGCCAGCCCGTTGCGGGTGGCGGCAGACGGTTCCGTCATCGCGCAAACGCCCACGCCTTCGGCGCCCGAGCCGATGTCC
GCCGCACCCTCGTCGTCTTCCTCAACAGAGAGTTCGGAAAGCGGCGAGCCCGCCAGCGTCGACGAATACAACGGCCTCGG
CTCCGATGCCAACGGCGATGCGTCGCAGTGGAACCTCGACGACTATGGCCGCTCCGGCAACGCGTCCGACGACGACGACC
TTCCGCCGCTGCAGATCCACGAATCCACCACGTCGCTGCGCGACTACCTGACAGCACAGTTGCGCGTCACGCAGGCAAGC
CCGCGTGACCGCGCGCTCATCACGTTCCTCATCGAATCGCTCGACGACGAAGGCTACCTCACGGCAACGTGTGAAGAAAT
ACTGGCCGACTTGCCAGTCGAGCTCGAGGTGGACGCCGACGAGTTGAATGCGGCACTCGCATTGCTGCAAAGCTTCGACC
CGCCTGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGTTGCTTCGGCTCGATCCGTCGCCTACTCGCACA
CTCGCGCTCGAAATCGTCGCGCACCATCTGGAGTTGCTTGCCGCACGAGATTTCACTCGTCTGCGCAAACATCTGAAGGC
AAGCGACGACGAACTGCGCGATGCCCATGCGCTGATCCGCTCGCTCGAACCGTTCCCGGGTGCGGCCTACGGCAAGGCCG
AAGCCGACTACGTCGTGCCCGACGTGATCGTGAAGAAGACCGCACAAGGATGGCACGCCGAACTGAATCCCGAGGTCGTG
CCGAAGCTGCGCATCAATCATCTGTACGCGAACATCCTGCGCAACAACCGCGGCGATCCAGGCAGCGGCTCGTTGCGTCA
GCAACTGCAGGAAGCCCGCTGGTTGATCAAAAATATTCAGCAGCGCTTCGAAACAATCCTGCGTGTTGCGCAGGCCATTG
TGGAGCGTCAGAAGAACTTTTTTGTGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCTTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTCACCCCGTTTGGGACGCTTGAATTTAAGTA
CTTCTTCGGATCGCATGTTTCGACGGATACGGGAGGCGCGGCCTCATCCACGGCCATACGCGCGCTCATCAAGCAACTGA
TAGGAGCAGAGAACCCGAAATCCCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTCGTGGTGGCG
CGCCGCACGGTGGCGAAGTATCGCGAGGCGCTCAAGATTCCAGCGGTCAACCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-329)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.127

100

0.681