Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LLUC147_RS10835 Genome accession   NZ_CP120473
Coordinates   2138748..2140109 (-) Length   453 a.a.
NCBI ID   WP_021164999.1    Uniprot ID   -
Organism   Lactococcus cremoris strain UC147     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2140279..2150031 2138748..2140109 flank 170
IS/Tn 2137757..2138647 2138748..2140109 flank 101


Gene organization within MGE regions


Location: 2137757..2150031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC147_RS10830 (LLUC147_10865) - 2137757..2138647 (+) 891 WP_014572485.1 IS982-like element IS982B family transposase -
  LLUC147_RS10835 (LLUC147_10870) radA 2138748..2140109 (-) 1362 WP_021164999.1 DNA repair protein RadA Machinery gene
  LLUC147_RS10840 (LLUC147_10875) - 2140279..2141295 (-) 1017 WP_396425231.1 glycosyltransferase family 2 protein -
  LLUC147_RS10845 (LLUC147_10880) - 2141277..2141822 (-) 546 WP_081196888.1 HAD-IIIA family hydrolase -
  LLUC147_RS10850 (LLUC147_10885) - 2141819..2142394 (-) 576 WP_081196889.1 SIS domain-containing protein -
  LLUC147_RS10855 (LLUC147_10890) - 2142456..2142632 (-) 177 WP_235853881.1 hypothetical protein -
  LLUC147_RS10860 (LLUC147_10895) - 2142608..2143186 (-) 579 WP_235853882.1 hypothetical protein -
  LLUC147_RS10865 (LLUC147_10900) - 2143395..2144177 (-) 783 WP_396425232.1 lipopolysaccharide biosynthesis protein -
  LLUC147_RS10870 (LLUC147_10905) - 2144598..2145545 (+) 948 WP_003130410.1 IS30 family transposase -
  LLUC147_RS10875 (LLUC147_10910) - 2145519..2145998 (-) 480 WP_396425233.1 hypothetical protein -
  LLUC147_RS10880 (LLUC147_10915) - 2145995..2146984 (-) 990 WP_061777883.1 alpha-1,2-fucosyltransferase -
  LLUC147_RS10885 (LLUC147_10920) - 2147236..2147916 (-) 681 WP_396425234.1 glycosyltransferase -
  LLUC147_RS10890 (LLUC147_10925) - 2147931..2148878 (-) 948 WP_003130410.1 IS30 family transposase -
  LLUC147_RS10895 (LLUC147_10930) - 2149051..2150031 (-) 981 WP_021164992.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49869.28 Da        Isoelectric Point: 5.2965

>NTDB_id=805798 LLUC147_RS10835 WP_021164999.1 2138748..2140109(-) (radA) [Lactococcus cremoris strain UC147]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFAEEVEVQEVKNQRVSMSGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIKVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=805798 LLUC147_RS10835 WP_021164999.1 2138748..2140109(-) (radA) [Lactococcus cremoris strain UC147]
ATGGCGAAGAAAAAATCATCTTTTGTTTGTCAAAATTGTGGTTATAAATCTGCAAAGTATCTTGGGCGATGCCCAAATTG
TGGAGAGTGGTCCTCTTTTGCTGAAGAAGTTGAAGTACAGGAAGTCAAAAATCAGCGAGTGTCGATGTCTGGTGAACGTT
CAAAACCAATGAAATTAGATGAAGTTGAGCTTTTTGATACACCACGTATTGAAACAGATCTTGACGAATTTAATCGTGTT
TTAGGTGGTGGTGTTGTTCCTGGTTCACTCGTTTTGATTGGTGGAGACCCAGGAATAGGAAAATCTACGTTGCTTTTGCA
AGTATCGACTCAGCTTGCATCAAGAGGACGGGTTCTCTATGTGAGTGGTGAAGAATCTGCTCAACAAATTAAGCTTAGAG
CGGAACGTTTGGGCGATATTGATCGAGATTTTTATCTTTACGCCGAGACTAACATGCAGTCAATTCGTGCTGAAGTTGAA
CGTTTGAAGCCAAATTTTTTGATTATTGATTCGATCCAAACTATTATGACACCTGAAATTCAATCAACTCAAGGCTCTGT
GAGTCAAGTTCGAGAAGTGACAGGTGAACTCATGCAAATTGCCAAAACGAATGATATTGCTACTTTTATCGTTGGTCATG
TGACGAAAGAAGGTCAATTGGCAGGACCAAGAATGCTAGAGCACATGGTTGATACCGTTCTTTATTTTGAAGGAGAAAGA
AATAATACTTTTAGAATTCTTCGAGCAGTTAAAAACCGGTTTGGGTCAACCAATGAAATTGGTATATTCGAAATGCAAGG
GCATGGTTTAGTTGAAGTAACTAATCCTTCTGAGGTTTTCTTGGAAGAGCGATTAGAGGGTTCAACTGGTTCTGCTATTG
TTTGTGCACTCGAAGGGACACGTCCGATATTAGTTGAAATACAGGCTTTAACTACACCAACAATGTTTGGAAATGCTAAG
AGAACAACTTCTGGTTTAGATTTTAATAGAGTGAGCCTGATTATGGCAGTACTTGAAAAACGGACAGGCTTATTGATGCA
AAATCAAGATGCTTACCTTAAATCAGCTGGGGGAGTAAAATTAGATGAGCCAGCCATTGACCTTGCAGTTGCTGTCGCTG
TAGCCTCAAGTTATAAAGAACTTCCGACGGATGCGCGTGAATGTTTTATTGGTGAAATTGGCTTAACAGGAGAGATTCGT
CGTGTGACGCGAATTGAACAAAGACTGAATGAAGCTGCTAAATTAGGATTCAAAAAAGTCTATGCTCCTAAAAATTCAAT
TGTGGGAATTGATATTCCTGAACAAATAAAAGTAATTGGGGTAACAACCCTAACTGAATGTCTAAAATTAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.795

100

0.808
  radA Streptococcus pneumoniae D39

80.795

100

0.808
  radA Streptococcus pneumoniae R6

80.795

100

0.808
  radA Streptococcus pneumoniae TIGR4

80.795

100

0.808
  radA Streptococcus mitis SK321

80.795

100

0.808
  radA Streptococcus mitis NCTC 12261

80.574

100

0.806
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62