Detailed information NTG0080514

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: L6499_RS25695
• Genome accession: NZ_AP022986
• Coordinates: 4941660..4943009 (-)
• Length: 449 a.a.
• NCBI ID: WP_000225368.1
• Uniprot ID: -
• Organism: Bacillus cereus strain 30052
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4936660..4948009

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6499_RS25675 (BC30052_5117)	secA	4936982..4939489 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
L6499_RS25680 (BC30052_5118)	raiA	4939766..4940308 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
L6499_RS25685 (BC30052_5119)	cspC	4940632..4940829 (-)	198	WP_001990088.1	cold shock protein CspC	-
L6499_RS25690 (BC30052_5120)	comFC	4940956..4941660 (-)	705	WP_041184339.1	comF operon protein ComFC	-
L6499_RS25695 (BC30052_5121)	comFA	4941660..4943009 (-)	1350	WP_000225368.1	ATP-dependent helicase ComFA	Machinery gene
L6499_RS25700 (BC30052_5122)	-	4943137..4944447 (-)	1311	WP_000734330.1	C40 family peptidase	-
L6499_RS25705 (BC30052_5123)	-	4944701..4945015 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
L6499_RS25710 (BC30052_5124)	-	4945189..4946031 (-)	843	WP_000844766.1	DegV family protein	-
L6499_RS25715 (BC30052_5125)	-	4946271..4946906 (+)	636	WP_000926684.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51268.96 Da
• Isoelectric Point: 9.7782

>NTDB_id=80514 L6499_RS25695 WP_000225368.1 4941660..4943009(-) (comFA) [Bacillus cereus strain 30052]
MVAGKQLLLEELSSDLRRELSDSKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCNKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=80514 L6499_RS25695 WP_000225368.1 4941660..4943009(-) (comFA) [Bacillus cereus strain 30052]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTCGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGTGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGGAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAATAAAGTATGTACGTATTGCCGCAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGTGGGATTCACGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGTTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCCGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACA
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTTC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAAGAGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAGGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

• Type III restriction enzyme, res subunit (ResIII) (108-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.843	96.659	0.501
comFA	Latilactobacillus sakei subsp. sakei 23K	40.75	89.087	0.363