Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L6499_RS21030 Genome accession   NZ_AP022986
Coordinates   4070735..4071778 (-) Length   347 a.a.
NCBI ID   WP_001013200.1    Uniprot ID   A0AA96SWA8
Organism   Bacillus cereus strain 30052     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4065735..4076778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6499_RS20985 (BC30052_4199) - 4066164..4066364 (-) 201 WP_000106084.1 YqzE family protein -
  L6499_RS20990 (BC30052_4200) aroK 4066403..4066900 (-) 498 WP_000836631.1 shikimate kinase AroK -
  L6499_RS20995 (BC30052_4201) - 4067021..4067671 (-) 651 WP_000183851.1 2OG-Fe(II) oxygenase -
  L6499_RS21000 comGG 4067847..4068216 (-) 370 Protein_4101 competence type IV pilus minor pilin ComGG -
  L6499_RS21005 (BC30052_4203) comGF 4068213..4068683 (-) 471 WP_000923089.1 competence type IV pilus minor pilin ComGF -
  L6499_RS21010 (BC30052_4204) comGE 4068653..4068955 (-) 303 WP_000829458.1 competence type IV pilus minor pilin ComGE -
  L6499_RS21015 (BC30052_4205) comGD 4068948..4069403 (-) 456 WP_000810393.1 comG operon protein ComGD -
  L6499_RS21020 (BC30052_4206) comGC 4069400..4069699 (-) 300 WP_001178696.1 comG operon protein ComGC -
  L6499_RS21025 (BC30052_4207) comGB 4069711..4070742 (-) 1032 WP_001995198.1 comG operon protein ComGB -
  L6499_RS21030 (BC30052_4208) comGA 4070735..4071778 (-) 1044 WP_001013200.1 competence protein ComGA Machinery gene
  L6499_RS21035 (BC30052_4209) - 4071984..4072679 (+) 696 WP_000434117.1 helix-turn-helix domain-containing protein -
  L6499_RS21040 (BC30052_4210) - 4072805..4073047 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  L6499_RS21045 (BC30052_4211) - 4073155..4074549 (+) 1395 WP_001094329.1 L-cystine transporter -
  L6499_RS21050 (BC30052_4212) - 4074766..4074981 (-) 216 WP_001008320.1 DUF3912 family protein -
  L6499_RS21055 (BC30052_4213) - 4075233..4075544 (+) 312 WP_001093243.1 hypothetical protein -
  L6499_RS21060 (BC30052_4214) - 4075580..4076068 (-) 489 WP_000764279.1 hypothetical protein -
  L6499_RS21065 (BC30052_4215) - 4076148..4076522 (-) 375 WP_001012350.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39412.97 Da        Isoelectric Point: 9.2147

>NTDB_id=80503 L6499_RS21030 WP_001013200.1 4070735..4071778(-) (comGA) [Bacillus cereus strain 30052]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=80503 L6499_RS21030 WP_001013200.1 4070735..4071778(-) (comGA) [Bacillus cereus strain 30052]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATATTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGTCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTCCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366

Multiple sequence alignment