Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P3U44_RS13405 Genome accession   NZ_CP120180
Coordinates   2638228..2639598 (-) Length   456 a.a.
NCBI ID   WP_049319440.1    Uniprot ID   A0AAW5LJW9
Organism   Mammaliicoccus sciuri strain Dog016     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2633228..2644598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U44_RS13380 (P3U44_13385) cysE 2633620..2634261 (-) 642 WP_025904850.1 serine O-acetyltransferase -
  P3U44_RS13385 (P3U44_13390) gltX 2634517..2635971 (-) 1455 WP_048541341.1 glutamate--tRNA ligase -
  P3U44_RS13390 (P3U44_13395) ispF 2635987..2636466 (-) 480 WP_049319442.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  P3U44_RS13395 (P3U44_13400) ispD 2636459..2637145 (-) 687 WP_070369128.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P3U44_RS13400 (P3U44_13405) - 2637147..2638205 (-) 1059 WP_048541336.1 PIN/TRAM domain-containing protein -
  P3U44_RS13405 (P3U44_13410) radA 2638228..2639598 (-) 1371 WP_049319440.1 DNA repair protein RadA Machinery gene
  P3U44_RS13410 (P3U44_13415) - 2639754..2642216 (-) 2463 WP_058590882.1 ATP-dependent Clp protease ATP-binding subunit -
  P3U44_RS13415 (P3U44_13420) - 2642233..2643240 (-) 1008 WP_049319439.1 protein arginine kinase -
  P3U44_RS13420 (P3U44_13425) - 2643241..2643777 (-) 537 WP_049319438.1 UvrB/UvrC motif-containing protein -
  P3U44_RS13425 (P3U44_13430) - 2643792..2644253 (-) 462 WP_025904841.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50463.21 Da        Isoelectric Point: 7.7103

>NTDB_id=804261 P3U44_RS13405 WP_049319440.1 2638228..2639598(-) (radA) [Mammaliicoccus sciuri strain Dog016]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEESIEHKETKGPKNTFKSDVPAQKIEKLKDVKEEDTPRIKTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTVFHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVSGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIVIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIIPKNNIKGLEFPDGTEVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=804261 P3U44_RS13405 WP_049319440.1 2638228..2639598(-) (radA) [Mammaliicoccus sciuri strain Dog016]
ATGGCCAAAAAGAAAGTAATATTTGAGTGTTTAGCCTGCGGATATCAATCTCCAAAATGGATGGGGAAATGCCCTAATTG
TGGTGCATGGAACCAAATGGAAGAATCAATAGAACATAAAGAAACTAAAGGTCCTAAAAATACATTCAAGTCAGATGTAC
CCGCACAAAAAATAGAAAAATTAAAAGACGTTAAAGAAGAAGATACACCTAGAATTAAGACTAAAATCAATGAATTTAAT
CGCGTATTAGGTGGCGGTGTTGTAGAAGGTTCGCTTGTACTTATTGGTGGTGACCCTGGTATTGGTAAATCCACATTATT
ATTACAAATTTGTGCGGCTTTATCTCAACAAAAAAATGTCTTATATGTTTCAGGTGAAGAATCTGTAAGACAAACGAAAT
TAAGAGCTGAAAGATTAGTTGAAGATTCAGGAGAATTAAATGTTTATGCTGAAACGAATTTAGAGATTATACATAGTATG
GTTCAAAAAGAAAAACCAGATGTATTAGTTATAGATTCCATTCAAACTGTCTTCCACCCTGAAGTTACTTCTGCTCCAGG
TTCTGTTTCTCAAGTTAGAGAATGTACACAAAATTTAATGCATATCGCAAAACAAATGAATATTGCTACATTTATTGTTG
GACATGTAACGAAGGATGGACAAATTGCTGGTCCACGTTTGTTAGAACATATGGTAGATACAGTATTATATTTTGAAGGC
GATACACATCATGCATACAGAATATTAAGAGCAGTTAAAAACCGTTTTGGTTCGACAAATGAAATGGGTATTTTTGAAAT
GAAGCAAACGGGTCTTAACGAAGTTAAAAATCCGTCTGAAATGTTCTTAGAAGAAAGAACGAAACATGTATCGGGTTCGA
CTATTGTGGCAACTATGGAAGGTACGAGACCTATTTTAGTTGAAGTGCAAGCTTTAGTAACGCCAACAACTTTTCATAAT
CCACGAAGAATGGCAACAGGTATTGATCACAATAGATTAAGCTTATTGATGGCTGTATTAGAGAAGAAAGAGAATTTCTT
ATTACAACAACAAGACGCATATATCAAAATTGCCGGTGGTGTAAAATTAAGTGAACCCGCTGTAGATTTAAGCATTGTTA
TCGCGATAGCTTCAAGTTTCCAAGATAAACCTACTAAAGGTGATGATTGTTTCGTGGGTGAGGTTGGCTTAACAGGTGAA
GTAAGAAGGGTTGCTAGAATTGATCAACGTGTACAAGAAGCGGCTAAATTAGGATTTAAACGTATTATCATACCTAAAAA
TAATATTAAAGGTTTAGAATTCCCAGATGGTACAGAAGTTATTGGCGTATCAACAACAAGAGAAGCATTGAAAATTGCTT
TTAATGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.721

100

0.66
  radA Streptococcus pneumoniae Rx1

61.894

99.561

0.616
  radA Streptococcus pneumoniae D39

61.894

99.561

0.616
  radA Streptococcus pneumoniae R6

61.894

99.561

0.616
  radA Streptococcus pneumoniae TIGR4

61.894

99.561

0.616
  radA Streptococcus mitis NCTC 12261

61.894

99.561

0.616
  radA Streptococcus mitis SK321

61.894

99.561

0.616