Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RJ648_RS18520 Genome accession   NZ_CP134380
Coordinates   3840410..3840850 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain TUM11353     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3835410..3845850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ648_RS18500 (RJ648_18505) aroP 3836408..3837781 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  RJ648_RS18505 (RJ648_18510) ampE 3837824..3838678 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  RJ648_RS18510 (RJ648_18515) ampD 3838675..3839226 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RJ648_RS18515 (RJ648_18520) nadC 3839314..3840207 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RJ648_RS18520 (RJ648_18525) pilA 3840410..3840850 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  RJ648_RS18525 (RJ648_18530) pilB 3840860..3842245 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  RJ648_RS18530 (RJ648_18535) hofC 3842235..3843437 (+) 1203 WP_000157234.1 protein transport protein HofC -
  RJ648_RS18535 (RJ648_18540) guaC 3843472..3844515 (-) 1044 WP_001217338.1 GMP reductase -
  RJ648_RS18540 (RJ648_18545) - 3844671..3844715 (-) 45 WP_120795372.1 protein YacM -
  RJ648_RS18545 (RJ648_18550) coaE 3844740..3845360 (+) 621 WP_001584165.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=803276 RJ648_RS18520 WP_000360895.1 3840410..3840850(+) (pilA) [Escherichia coli strain TUM11353]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=803276 RJ648_RS18520 WP_000360895.1 3840410..3840850(+) (pilA) [Escherichia coli strain TUM11353]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363