Detailed information NTG0080318

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: L6478_RS25895
• Genome accession: NZ_AP022970
• Coordinates: 4982564..4983913 (-)
• Length: 449 a.a.
• NCBI ID: WP_236787299.1
• Uniprot ID: -
• Organism: Bacillus cereus strain J75
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4977564..4988913

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6478_RS25875 (BCJMU75_5112)	secA	4977886..4980393 (-)	2508	WP_000579368.1	preprotein translocase subunit SecA	-
L6478_RS25880 (BCJMU75_5113)	raiA	4980670..4981212 (-)	543	WP_236795830.1	ribosome-associated translation inhibitor RaiA	-
L6478_RS25885 (BCJMU75_5114)	cspC	4981536..4981733 (-)	198	WP_033656419.1	cold shock protein CspC	-
L6478_RS25890 (BCJMU75_5115)	comFC	4981860..4982564 (-)	705	WP_062921932.1	comF operon protein ComFC	-
L6478_RS25895 (BCJMU75_5116)	comFA	4982564..4983913 (-)	1350	WP_236787299.1	ATP-dependent helicase ComFA	Machinery gene
L6478_RS25900 (BCJMU75_5117)	-	4984041..4985469 (-)	1429	Protein_5058	NlpC/P60 family protein	-
L6478_RS25905 (BCJMU75_5118)	-	4985617..4985931 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
L6478_RS25910 (BCJMU75_5119)	-	4986105..4986947 (-)	843	WP_000844766.1	DegV family protein	-
L6478_RS25915 (BCJMU75_5120)	-	4987187..4987822 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51305.95 Da
• Isoelectric Point: 9.5085

>NTDB_id=80318 L6478_RS25895 WP_236787299.1 4982564..4983913(-) (comFA) [Bacillus cereus strain J75]
MVAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGIIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEISLLLKELDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=80318 L6478_RS25895 WP_236787299.1 4982564..4983913(-) (comFA) [Bacillus cereus strain J75]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGTGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGGAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTATTATAGAAGCTATTAAGCAGAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTTC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCACCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAAGCCTGTTATTGAAAGAGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

• Type III restriction enzyme, res subunit (ResIII) (108-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.675	93.096	0.481