Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RJ642_RS02060 Genome accession   NZ_CP134370
Coordinates   446455..447132 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain TUM12357     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441455..452132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ642_RS02045 (RJ642_02045) chiA 442822..445515 (+) 2694 WP_310995131.1 bifunctional chitinase/lysozyme -
  RJ642_RS02050 (RJ642_02050) bfd 445684..445878 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RJ642_RS02055 (RJ642_02055) bfr 445950..446426 (+) 477 WP_000675504.1 bacterioferritin -
  RJ642_RS02060 (RJ642_02060) pilD 446455..447132 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  RJ642_RS02065 (RJ642_02065) gspM 447132..447593 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RJ642_RS02070 (RJ642_02070) gspL 447590..448753 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  RJ642_RS02075 (RJ642_02075) gspK 448768..449751 (-) 984 WP_157701142.1 type II secretion system minor pseudopilin GspK -
  RJ642_RS02080 (RJ642_02080) gspJ 449744..450331 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RJ642_RS02085 (RJ642_02085) gspI 450324..450701 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RJ642_RS02090 (RJ642_02090) gspH 450698..451207 (-) 510 WP_248788947.1 type II secretion system minor pseudopilin GspH -
  RJ642_RS02095 (RJ642_02095) gspG 451215..451652 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=803070 RJ642_RS02060 WP_000178154.1 446455..447132(-) (pilD) [Escherichia coli strain TUM12357]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=803070 RJ642_RS02060 WP_000178154.1 446455..447132(-) (pilD) [Escherichia coli strain TUM12357]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378