Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L6478_RS21035 Genome accession   NZ_AP022970
Coordinates   4054306..4055349 (-) Length   347 a.a.
NCBI ID   WP_001013193.1    Uniprot ID   -
Organism   Bacillus cereus strain J75     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4049306..4060349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6478_RS20990 (BCJMU75_4149) - 4049733..4049933 (-) 201 WP_000106084.1 YqzE family protein -
  L6478_RS20995 (BCJMU75_4150) aroK 4049972..4050469 (-) 498 WP_000836631.1 shikimate kinase AroK -
  L6478_RS21000 (BCJMU75_4151) - 4050590..4051240 (-) 651 WP_000183880.1 2OG-Fe(II) oxygenase -
  L6478_RS21005 (BCJMU75_4152) comGG 4051416..4051787 (-) 372 WP_001231191.1 competence type IV pilus minor pilin ComGG -
  L6478_RS21010 (BCJMU75_4153) comGF 4051784..4052254 (-) 471 WP_236788657.1 competence type IV pilus minor pilin ComGF -
  L6478_RS21015 (BCJMU75_4154) comGE 4052224..4052526 (-) 303 WP_000829458.1 competence type IV pilus minor pilin ComGE -
  L6478_RS21020 (BCJMU75_4155) comGD 4052519..4052974 (-) 456 WP_000810393.1 comG operon protein ComGD -
  L6478_RS21025 (BCJMU75_4156) comGC 4052971..4053270 (-) 300 WP_001178696.1 comG operon protein ComGC -
  L6478_RS21030 (BCJMU75_4157) comGB 4053282..4054313 (-) 1032 WP_072192778.1 comG operon protein ComGB -
  L6478_RS21035 (BCJMU75_4158) comGA 4054306..4055349 (-) 1044 WP_001013193.1 competence protein ComGA Machinery gene
  L6478_RS21040 (BCJMU75_4159) - 4055554..4056249 (+) 696 WP_000434132.1 helix-turn-helix domain-containing protein -
  L6478_RS21045 (BCJMU75_4160) - 4056374..4056616 (+) 243 WP_000440720.1 DUF2626 domain-containing protein -
  L6478_RS21050 (BCJMU75_4161) - 4056724..4058118 (+) 1395 WP_001094341.1 L-cystine transporter -
  L6478_RS21055 (BCJMU75_4162) - 4058335..4058550 (-) 216 WP_001008320.1 DUF3912 family protein -
  L6478_RS21060 (BCJMU75_4163) - 4058803..4059114 (+) 312 WP_001093243.1 hypothetical protein -
  L6478_RS21065 (BCJMU75_4164) - 4059151..4059639 (-) 489 WP_236787095.1 hypothetical protein -
  L6478_RS21070 (BCJMU75_4165) - 4059719..4060093 (-) 375 WP_001012359.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39377.92 Da        Isoelectric Point: 9.2320

>NTDB_id=80307 L6478_RS21035 WP_001013193.1 4054306..4055349(-) (comGA) [Bacillus cereus strain J75]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=80307 L6478_RS21035 WP_001013193.1 4054306..4055349(-) (comGA) [Bacillus cereus strain J75]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAGGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGGTTACTCGTATTTACTGGACCGACTGGTTCAGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGCCTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

36.261

100

0.369

Multiple sequence alignment