Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P3T86_RS12295 Genome accession   NZ_CP120099
Coordinates   2548431..2549801 (-) Length   456 a.a.
NCBI ID   WP_096810933.1    Uniprot ID   A0A291JNJ4
Organism   Staphylococcus nepalensis strain Dog109     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2543431..2554801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T86_RS12280 (P3T86_12280) cysE 2544430..2545071 (-) 642 WP_096811242.1 serine O-acetyltransferase -
  P3T86_RS12285 (P3T86_12285) gltX 2545412..2546866 (-) 1455 WP_119487751.1 glutamate--tRNA ligase -
  P3T86_RS12290 (P3T86_12290) - 2547299..2548357 (-) 1059 WP_103372873.1 PIN/TRAM domain-containing protein -
  P3T86_RS12295 (P3T86_12295) radA 2548431..2549801 (-) 1371 WP_096810933.1 DNA repair protein RadA Machinery gene
  P3T86_RS12300 (P3T86_12300) - 2550152..2552614 (-) 2463 WP_096810934.1 ATP-dependent Clp protease ATP-binding subunit -
  P3T86_RS12305 (P3T86_12305) - 2552626..2553636 (-) 1011 WP_170006919.1 protein arginine kinase -
  P3T86_RS12310 (P3T86_12310) - 2553629..2554180 (-) 552 WP_096810936.1 UvrB/UvrC motif-containing protein -
  P3T86_RS12315 (P3T86_12315) - 2554197..2554658 (-) 462 WP_096810937.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50332.90 Da        Isoelectric Point: 7.7474

>NTDB_id=802954 P3T86_RS12295 WP_096810933.1 2548431..2549801(-) (radA) [Staphylococcus nepalensis strain Dog109]
MAKKKVIFECMACGYQSPKWMGKCPNCGGWNQMEEIIEQKSTSPKHGVRSRENSAKVQKLNEIKHETTPRIKTNSAEFNR
VLGGGIVNGSLVLIGGDPGIGKSTLLLQICAALSQSKSVLYITGEESLNQTKLRADRLEEDSSNLNVFAETDLEVIHETV
KQLKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQLNIPTFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIVIATASSFKDLTVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAEKLGFKRIIIPESNIGGWEFPSGIKVIGVKNVHEALKYALTKH

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=802954 P3T86_RS12295 WP_096810933.1 2548431..2549801(-) (radA) [Staphylococcus nepalensis strain Dog109]
TTGGCCAAAAAGAAAGTGATTTTTGAATGTATGGCGTGTGGTTATCAATCACCAAAATGGATGGGCAAATGTCCCAACTG
TGGTGGTTGGAACCAAATGGAAGAAATTATAGAACAAAAATCGACAAGCCCAAAACATGGGGTTCGAAGTCGTGAAAATA
GCGCGAAAGTACAAAAACTAAATGAAATTAAACATGAGACGACACCACGTATTAAAACCAACTCAGCAGAATTTAACCGA
GTACTTGGCGGTGGCATTGTAAACGGTTCGCTTGTATTAATTGGCGGCGACCCAGGTATTGGTAAATCGACCTTATTACT
ACAAATTTGTGCTGCTTTATCACAAAGTAAAAGCGTTTTATATATAACAGGTGAAGAGTCGTTAAATCAAACTAAGTTAA
GAGCAGATAGATTAGAAGAGGATTCAAGTAATTTAAATGTGTTTGCGGAAACAGATTTAGAAGTGATACATGAAACGGTT
AAACAATTGAAACCAGATTTACTTGTAGTTGATTCTATACAGACTATTTTTCATCCTGAAATCAGCTCTGCGCCTGGTTC
AGTATCTCAAGTAAGAGAAAGTACACAGAGCTTAATGAATATTGCTAAACAATTAAACATCCCAACTTTTATTGTTGGTC
ATGTAACTAAAGAAGGGCAAATTGCAGGTCCACGTTTATTAGAACATATGGTGGATACAGTACTTTATTTTGAAGGTGAT
GAGCATCATGCTTACCGTATTTTAAGAGCAGTTAAAAATCGCTTTGGTTCGACAAATGAAATGGGTATTTTTGAAATGAA
GCAGAGTGGATTGAAAGGCGTGAAAAACCCTTCAGAAATGTTTTTGGAAGAGCGTTCTACTAATGTTCCTGGGTCAACAA
TCGTATCGACAATGGAAGGCACAAGACCATTGCTCATAGAAGTACAAGCATTAGTGACACCGACCACATTTAACAACCCA
AGACGTATGGCTACAGGCATAGACCATAATCGATTAAGTTTATTGATGGCTGTCTTAGAGAAAAAAGAAAATTATTTGCT
TCAACAACAAGATGCTTATATTAAAGTAGCAGGTGGCGTCAGATTAACAGAACCTGCTGTTGATTTAGGTATTGTTATTG
CCACGGCGTCTAGTTTTAAAGATTTAACTGTAGACGGATTGGATTGCTTTATTGGTGAAGTTGGATTGACGGGAGAAGTA
AGACGCGTCTCTCGTATCGAACAACGCGTACAAGAAGCGGAAAAACTAGGATTTAAACGTATTATTATTCCTGAATCCAA
TATTGGTGGATGGGAATTCCCTAGCGGTATTAAAGTCATTGGTGTTAAAAATGTACATGAGGCTTTAAAATATGCTTTAA
CTAAGCATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(356-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291JNJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

100

0.658
  radA Streptococcus pneumoniae Rx1

59.111

98.684

0.583
  radA Streptococcus pneumoniae D39

59.111

98.684

0.583
  radA Streptococcus pneumoniae R6

59.111

98.684

0.583
  radA Streptococcus pneumoniae TIGR4

59.111

98.684

0.583
  radA Streptococcus mitis NCTC 12261

59.111

98.684

0.583
  radA Streptococcus mitis SK321

59.111

98.684

0.583