Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   L6478_RS06225 Genome accession   NZ_AP022970
Coordinates   1205547..1207373 (+) Length   608 a.a.
NCBI ID   WP_062922370.1    Uniprot ID   A0AAE4TRC7
Organism   Bacillus cereus strain J75     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1200547..1212373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6478_RS06205 (BCJMU75_1140) - 1200869..1201543 (-) 675 WP_000362617.1 TerC family protein -
  L6478_RS06210 (BCJMU75_1141) mecA 1201870..1202553 (+) 684 WP_000350720.1 adaptor protein MecA Regulator
  L6478_RS06215 (BCJMU75_1142) - 1202626..1204170 (+) 1545 WP_062922372.1 cardiolipin synthase -
  L6478_RS06220 (BCJMU75_1143) - 1204252..1205496 (+) 1245 WP_236787817.1 competence protein CoiA family protein -
  L6478_RS06225 (BCJMU75_1144) pepF 1205547..1207373 (+) 1827 WP_062922370.1 oligoendopeptidase F Regulator
  L6478_RS06230 (BCJMU75_1145) - 1207493..1207666 (-) 174 WP_002051510.1 hypothetical protein -
  L6478_RS06235 (BCJMU75_1146) - 1207896..1208789 (-) 894 WP_000360638.1 ClpXP adapter SpxH family protein -
  L6478_RS06240 (BCJMU75_1147) - 1208789..1209187 (-) 399 WP_000043381.1 hypothetical protein -
  L6478_RS06245 (BCJMU75_1148) - 1209368..1209946 (-) 579 WP_000191105.1 CYTH domain-containing protein -
  L6478_RS06250 (BCJMU75_1149) - 1210094..1210465 (+) 372 WP_062922369.1 hypothetical protein -
  L6478_RS06255 (BCJMU75_1150) - 1210496..1211134 (+) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  L6478_RS06260 (BCJMU75_1151) - 1211153..1211950 (+) 798 WP_016088884.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70290.57 Da        Isoelectric Point: 4.6458

>NTDB_id=80291 L6478_RS06225 WP_062922370.1 1205547..1207373(+) (pepF) [Bacillus cereus strain J75]
MSEQNTAKTLPDRNEIEESSTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSANNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVYETYGKYKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVESVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDMLLKSLHVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=80291 L6478_RS06225 WP_062922370.1 1205547..1207373(+) (pepF) [Bacillus cereus strain J75]
ATGTCTGAACAAAATACAGCAAAAACATTACCAGATCGTAACGAGATTGAAGAATCAAGTACGTGGAGATTGGAAGATAT
TTTCCAAACGGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGAACAATCTACTTGAAGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCGTTAAATGACCGCGCGACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCATATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTAA
AAGAAAATAGAGACTTAAGTGTATATGAACATGCATTAGAAGAAATTACGCGCCAACGTCCGCACGTATTATCTGAAGCG
GAAGAGGCTTTATTAGCAGAAGCATCTGAAGTAATGAGTGCGTCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAAGTAGAGATTACACACGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTGCGCGAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATATAAGAATACATTTGCAAGTACG
TTAAGCGGAGCTGTGAAACGTAATAATTTTAATGCACGCGTTCGTAAATACGACTCTGCACGTCAAGCGGCATTAAGTAA
TAATAATATTCCTGAAGCGGTGTACGATCAACTTGTTGAATCTGTAAATGATAATTTACACTTGTTACATCGTTACATTG
ATATTCGTAAGCGTGCACTAGGGCTTGATGAGCTTCATATGTATGATTTATATACACCACTCGTACCAGAAGTGAAAATG
AACGTGAAGTATGAAGAAGCGCAAGACATGTTATTAAAGTCTTTACATGTACTTGGTGATGAGTATGTTGATATTTTAAA
AGAAGCATATGAAAATCGTTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGAGCATATTCATCTGGTGCATATG
GAACAAATCCTTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAATTTGGTCATTCT
GTGCATAGTTACTATACAAGAAAAACGCAGCCGCACGTATACGGTGACTATTCAATCTTTGTTGCGGAAGTAGCATCAAC
CTGTAATGAGGCGTTGCTAAACGATTACTTATTAAAAACGACAGAAGATAAGAAAGAACGTCTATACTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTCGCAGAGTTTGAGCATATTATTCATAAGAAAGTACAA
GAGGGACATGCGGTTACGCCAGACATGTTAACGGAGATCTACTATGATTTAAATAAGAAATATTTCGGTGATGCTTTAGT
CATTGATGAAGAAATTGGTTTAGAGTGGTCACGTATTCCGCACTTCTACTACAACTATTACGTATACCAATACGCAACAG
GATTTAGTGCAGCAACTGCTCTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGCTATATTAATGAGTTC
TTAAAGGCAGGAAGTTCGGATTATCCAATTGAAGTGCTGAAAAAAGCTGGAGTAGATATGGCATCTCCAGAACCGGTAAA
AGAAGCATTGCAAGTGTTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAGAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.253

97.533

0.49

Multiple sequence alignment