Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RJ647_RS02045 Genome accession   NZ_CP134353
Coordinates   455259..455936 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain TUM17668     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450259..460936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ647_RS02030 (RJ647_02030) chiA 451626..454319 (+) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -
  RJ647_RS02035 (RJ647_02035) bfd 454488..454682 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RJ647_RS02040 (RJ647_02040) bfr 454754..455230 (+) 477 WP_000675504.1 bacterioferritin -
  RJ647_RS02045 (RJ647_02045) pilD 455259..455936 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RJ647_RS02050 (RJ647_02050) - 456043..457205 (+) 1163 WP_085947771.1 IS3-like element IS3 family transposase -
  RJ647_RS02055 (RJ647_02055) gspM 457233..457658 (-) 426 WP_086977277.1 type II secretion system protein GspM -
  RJ647_RS02060 (RJ647_02060) gspL 457655..458818 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  RJ647_RS02065 (RJ647_02065) gspK 458833..459816 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  RJ647_RS02070 (RJ647_02070) gspJ 459809..460396 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RJ647_RS02075 (RJ647_02075) gspI 460389..460766 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=802736 RJ647_RS02045 WP_000178161.1 455259..455936(-) (pilD) [Escherichia coli strain TUM17668]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=802736 RJ647_RS02045 WP_000178161.1 455259..455936(-) (pilD) [Escherichia coli strain TUM17668]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378