Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P3U77_RS10920 Genome accession   NZ_CP120082
Coordinates   2299426..2300796 (-) Length   456 a.a.
NCBI ID   WP_096533775.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain Dog117_P     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2294426..2305796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U77_RS10895 (P3U77_10895) cysE 2294591..2295256 (-) 666 WP_172425792.1 serine O-acetyltransferase -
  P3U77_RS10900 (P3U77_10900) gltX 2295644..2297098 (-) 1455 WP_037543943.1 glutamate--tRNA ligase -
  P3U77_RS10905 (P3U77_10905) ispF 2297175..2297654 (-) 480 WP_014614766.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  P3U77_RS10910 (P3U77_10910) ispD 2297647..2298345 (-) 699 WP_014614767.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P3U77_RS10915 (P3U77_10915) - 2298346..2299407 (-) 1062 WP_014614768.1 PIN/TRAM domain-containing protein -
  P3U77_RS10920 (P3U77_10920) radA 2299426..2300796 (-) 1371 WP_096533775.1 DNA repair protein RadA Machinery gene
  P3U77_RS10925 (P3U77_10925) - 2301183..2301929 (-) 747 WP_015728605.1 ABC transporter permease -
  P3U77_RS10930 (P3U77_10930) - 2301916..2302620 (-) 705 WP_014614771.1 ABC transporter ATP-binding protein -
  P3U77_RS10935 (P3U77_10935) - 2302635..2303594 (-) 960 WP_225549892.1 hypothetical protein -
  P3U77_RS10940 (P3U77_10940) - 2303594..2304406 (-) 813 WP_063278650.1 SagB family peptide dehydrogenase -
  P3U77_RS10945 (P3U77_10945) - 2304426..2305646 (-) 1221 WP_014614774.1 YcaO-like family protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50010.48 Da        Isoelectric Point: 7.0051

>NTDB_id=802694 P3U77_RS10920 WP_096533775.1 2299426..2300796(-) (radA) [Staphylococcus pseudintermedius strain Dog117_P]
MAKTKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTASPKHGVRAQKTSSAKIQKLDDIKQELAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKEKRVLYITGEESLNQTKIRADRLDEDASQLNVFAETDLMVIHEA
VKKVEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNVATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERTTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVSGGVKLSEPAVDLGIVIATASSFKDQAVDGLDCYIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWHFPEGIEVIGVTSVNEALKYALKN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=802694 P3U77_RS10920 WP_096533775.1 2299426..2300796(-) (radA) [Staphylococcus pseudintermedius strain Dog117_P]
GTGGCAAAAACAAAAACAATTTTTGAATGTACGGCCTGTGGTTATCAATCGCCTAAATGGATGGGGAAATGTCCAAATTG
TGGTGCTTGGAATTCAATGGAGGAATCTTTTGAACAAAAAACTGCGAGTCCTAAACACGGTGTACGGGCGCAAAAAACAA
GTTCAGCAAAAATACAAAAGCTCGATGACATTAAACAAGAGCTTGCGCCACGTATCCAAACGAATAGTGGTGAATTAAAC
CGTGTGCTCGGTGGCGGTATTGTAGAAGGGTCACTCGTGCTGATTGGTGGCGATCCAGGAATAGGGAAGTCCACATTGTT
ACTTCAAATGTGCGCGGCTTTATCTAAAGAGAAAAGGGTGCTCTATATTACTGGTGAGGAATCACTCAATCAAACTAAAA
TTAGAGCGGATCGTTTGGATGAAGATGCGAGTCAACTCAACGTGTTTGCGGAAACGGACTTAATGGTTATTCATGAAGCG
GTGAAAAAGGTCGAACCTGATTTAATTGTAGTGGACTCTATTCAGACGATCTACCATCCCGAAATCAGTTCGGCGCCTGG
CTCTGTATCTCAAGTTCGTGAAAGTACACAAAGCTTGATGCACATCGCTAAACAAATGAATGTTGCGACGTTTATTGTAG
GCCATGTGACGAAAGAAGGCCAAATCGCGGGGCCGCGTTTACTTGAACATATGGTGGATACGGTGCTTTATTTTGAAGGT
GATGAACATCATGCGTATCGTATTTTAAGAGCGGTGAAAAACCGTTTTGGCTCTACGAATGAAATGGGTATTTTTGAAAT
GAAACAGTCAGGTTTAAAAAGCGTGCTTAACCCGTCAGAAATGTTTTTAGAAGAACGGACGACCAATGTTGCAGGTTCGA
CAATTGTCGCAACGATGGAAGGGACACGACCATTGTTAATTGAAGTACAAGCGCTTGTCACACCGACGACGTTCAATAAT
CCGAGACGTATGGCGACAGGTATTGACCATAATCGCCTCAGTTTGTTAATGGCGGTGTTAGAGAAAAAAGAAGGTTATTT
ATTACAACAACAAGACGCTTACATTAAGGTGTCTGGGGGCGTCAAACTCTCTGAACCTGCTGTCGACTTAGGTATCGTCA
TTGCGACAGCTTCTAGTTTTAAAGACCAAGCAGTAGACGGCCTCGATTGTTATATTGGCGAGGTTGGATTGACTGGTGAG
GTCCGCCGTGTTTCGCGCATTGAGCAACGTGTGCAAGAAGCAGCAAAATTGGGCTTTAAACGTGTGATTATCCCTAAAAA
CAATATTGGCGGATGGCACTTCCCAGAAGGTATTGAAGTGATTGGCGTGACTTCTGTCAATGAAGCATTGAAATACGCAT
TAAAAAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(350-428)

AAA domain (AAA_25)

(71-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.57

100

0.656
  radA Streptococcus pneumoniae Rx1

58.407

99.123

0.579
  radA Streptococcus pneumoniae D39

58.407

99.123

0.579
  radA Streptococcus pneumoniae R6

58.407

99.123

0.579
  radA Streptococcus pneumoniae TIGR4

58.407

99.123

0.579
  radA Streptococcus mitis NCTC 12261

58.407

99.123

0.579
  radA Streptococcus mitis SK321

58.407

99.123

0.579