Detailed information NTG0080268

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: L6484_RS26560
• Genome accession: NZ_AP022964
• Coordinates: 5132045..5133394 (-)
• Length: 449 a.a.
• NCBI ID: WP_061531556.1
• Uniprot ID: -
• Organism: Bacillus cereus strain J62
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5127045..5138394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6484_RS26540 (BCJMU62_5280)	secA	5127367..5129874 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
L6484_RS26545 (BCJMU62_5281)	raiA	5130151..5130693 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
L6484_RS26550 (BCJMU62_5282)	cspC	5131017..5131214 (-)	198	WP_001990088.1	cold shock protein CspC	-
L6484_RS26555 (BCJMU62_5283)	comFC	5131341..5132045 (-)	705	WP_041184339.1	comF operon protein ComFC	-
L6484_RS26560 (BCJMU62_5284)	comFA	5132045..5133394 (-)	1350	WP_061531556.1	ATP-dependent helicase ComFA	Machinery gene
L6484_RS26565 (BCJMU62_5285)	-	5133522..5134832 (-)	1311	WP_000734330.1	C40 family peptidase	-
L6484_RS26570 (BCJMU62_5286)	-	5135086..5135400 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
L6484_RS26575 (BCJMU62_5287)	-	5135574..5136416 (-)	843	WP_000844766.1	DegV family protein	-
L6484_RS26580 (BCJMU62_5288)	-	5136656..5137291 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51370.06 Da
• Isoelectric Point: 9.7210

>NTDB_id=80268 L6484_RS26560 WP_061531556.1 5132045..5133394(-) (comFA) [Bacillus cereus strain J62]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=80268 L6484_RS26560 WP_061531556.1 5132045..5133394(-) (comFA) [Bacillus cereus strain J62]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATTATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAGGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

• Type III restriction enzyme, res subunit (ResIII) (107-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.5	89.087	0.361