Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   L6484_RS20185 Genome accession   NZ_AP022964
Coordinates   3971110..3971544 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus cereus strain J62     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3966110..3976544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6484_RS20170 (BCJMU62_4016) - 3966874..3967431 (+) 558 WP_000062080.1 PadR family transcriptional regulator -
  L6484_RS20175 (BCJMU62_4017) metE 3968359..3970647 (+) 2289 WP_001007632.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  L6484_RS20180 (BCJMU62_4018) comJ 3970684..3971073 (-) 390 WP_000424033.1 competence protein ComJ -
  L6484_RS20185 (BCJMU62_4019) nucA/comI 3971110..3971544 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  L6484_RS20190 (BCJMU62_4020) - 3971781..3972530 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  L6484_RS20195 (BCJMU62_4021) kinB 3972584..3973858 (-) 1275 WP_000420389.1 sporulation sensor histidine kinase KinB -
  L6484_RS20200 (BCJMU62_4022) - 3974169..3974369 (+) 201 WP_000929284.1 hypothetical protein -
  L6484_RS20205 (BCJMU62_4023) - 3974492..3975655 (+) 1164 WP_000434590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=80254 L6484_RS20185 WP_000811492.1 3971110..3971544(-) (nucA/comI) [Bacillus cereus strain J62]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=80254 L6484_RS20185 WP_000811492.1 3971110..3971544(-) (nucA/comI) [Bacillus cereus strain J62]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment