Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RHT75_RS02190 Genome accession   NZ_CP133927
Coordinates   471775..472452 (-) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain M22546     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466775..477452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT75_RS02175 (RHT75_02175) chiA 468133..470835 (+) 2703 WP_311123123.1 bifunctional chitinase/lysozyme -
  RHT75_RS02180 (RHT75_02180) bfd 471004..471198 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RHT75_RS02185 (RHT75_02185) bfr 471270..471746 (+) 477 WP_000675504.1 bacterioferritin -
  RHT75_RS02190 (RHT75_02190) pilD 471775..472452 (-) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  RHT75_RS02195 (RHT75_02195) gspM 472452..472913 (-) 462 WP_001296465.1 type II secretion system protein GspM -
  RHT75_RS02200 (RHT75_02200) gspL 472910..474073 (-) 1164 WP_001296464.1 type II secretion system protein GspL -
  RHT75_RS02205 (RHT75_02205) gspK 474088..475071 (-) 984 WP_311123124.1 type II secretion system minor pseudopilin GspK -
  RHT75_RS02210 (RHT75_02210) gspJ 475064..475651 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  RHT75_RS02215 (RHT75_02215) gspI 475644..476021 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  RHT75_RS02220 (RHT75_02220) gspH 476018..476527 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  RHT75_RS02225 (RHT75_02225) gspG 476535..476972 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=801799 RHT75_RS02190 WP_000178170.1 471775..472452(-) (pilD) [Escherichia coli strain M22546]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=801799 RHT75_RS02190 WP_000178170.1 471775..472452(-) (pilD) [Escherichia coli strain M22546]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382