Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   RHT74_RS22985 Genome accession   NZ_CP133923
Coordinates   4708939..4711020 (+) Length   693 a.a.
NCBI ID   WP_000678405.1    Uniprot ID   -
Organism   Escherichia coli strain M21816     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4703939..4716020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT74_RS22965 (RHT74_22965) gmk 4705157..4705780 (+) 624 WP_001295237.1 guanylate kinase -
  RHT74_RS22970 (RHT74_22970) rpoZ 4705835..4706110 (+) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  RHT74_RS22975 (RHT74_22975) spoT 4706129..4708237 (+) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  RHT74_RS22980 (RHT74_22980) trmH 4708244..4708933 (+) 690 WP_001070179.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  RHT74_RS22985 (RHT74_22985) recG 4708939..4711020 (+) 2082 WP_000678405.1 ATP-dependent DNA helicase RecG Machinery gene
  RHT74_RS22990 (RHT74_22990) gltS 4711054..4712259 (-) 1206 WP_000468826.1 sodium/glutamate symporter -
  RHT74_RS22995 (RHT74_22995) xanP 4712539..4713930 (+) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  RHT74_RS23000 (RHT74_23000) yicH 4714051..4715760 (+) 1710 WP_001576397.1 AsmA family protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76524.50 Da        Isoelectric Point: 7.3384

>NTDB_id=801775 RHT74_RS22985 WP_000678405.1 4708939..4711020(+) (recG) [Escherichia coli strain M21816]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=801775 RHT74_RS22985 WP_000678405.1 4708939..4711020(+) (recG) [Escherichia coli strain M21816]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTACAGGATTTGCTCTTACACCTTCCTCTGCGCTACGAAGATCGCACACATCTCTACCCCATCG
GTGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACTATGCGCTTTTTCAACTTCAATGCGGCAATGAAAAATAGCCT
GGCGACAGGCCGCCGCGTGCTGGCCTATGGCGAAGCAAAGCGCGGCAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTACAGGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCTACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACATGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCACCGCCGACGCTACAGCTTAGCGATCTCG
AAACCGGGCAGCATCCGGCACAACGCCGGCTGATTCTGGAAGAGTTGCTGGCGCACAACCTCAGCATGTTGGCGTTACGT
GCCGGTGCACAACGTTTTCATGCTCAGCCGCTGAGCGCCAATGACGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCGCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTAGGCTGGCTCGCCGGAAAACAGAAAGGTAAAGCACGACTGGCTCAGCAAGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCCATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCACCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGTATG
ACTGAAGGGCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTACTGGAAGCGCAGGCGGCGGAAGCGAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGACGGATGAAACCCGCCGAGAAACAGG
CGGTGATGACATCGTTTAAACAAGGCGAGCTACACCTGCTGGTTGCCACTACTGTCATTGAAGTCGGTGTTGATGTACCC
AATGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATCCGCCTGCAGGTACTAC
GCGACAGCAACGATGGTTTTGTGATTGCACAGAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGTACACGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.21

94.949

0.372