Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RHT74_RS21360 Genome accession   NZ_CP133923
Coordinates   4356561..4357238 (+) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain M21816     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4351561..4362238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT74_RS21325 (RHT74_21325) gspG 4352041..4352478 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  RHT74_RS21330 (RHT74_21330) gspH 4352486..4352995 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  RHT74_RS21335 (RHT74_21335) gspI 4352992..4353369 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  RHT74_RS21340 (RHT74_21340) gspJ 4353362..4353949 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  RHT74_RS21345 (RHT74_21345) gspK 4353942..4354925 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  RHT74_RS21350 (RHT74_21350) gspL 4354940..4356103 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  RHT74_RS21355 (RHT74_21355) gspM 4356100..4356561 (+) 462 WP_001296465.1 type II secretion system protein GspM -
  RHT74_RS21360 (RHT74_21360) pilD 4356561..4357238 (+) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  RHT74_RS21365 (RHT74_21365) bfr 4357267..4357743 (-) 477 WP_000675504.1 bacterioferritin -
  RHT74_RS21370 (RHT74_21370) bfd 4357815..4358009 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RHT74_RS21375 (RHT74_21375) chiA 4358178..4360880 (-) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=801766 RHT74_RS21360 WP_000178170.1 4356561..4357238(+) (pilD) [Escherichia coli strain M21816]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=801766 RHT74_RS21360 WP_000178170.1 4356561..4357238(+) (pilD) [Escherichia coli strain M21816]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382