Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RHT74_RS03720 Genome accession   NZ_CP133923
Coordinates   788647..789087 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain M21816     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783647..794087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT74_RS03695 (RHT74_03695) coaE 784137..784757 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  RHT74_RS03700 (RHT74_03700) - 784782..784826 (+) 45 WP_120795372.1 protein YacM -
  RHT74_RS03705 (RHT74_03705) guaC 784982..786025 (+) 1044 WP_001217320.1 GMP reductase -
  RHT74_RS03710 (RHT74_03710) hofC 786060..787262 (-) 1203 WP_000157289.1 protein transport protein HofC -
  RHT74_RS03715 (RHT74_03715) pilB 787252..788637 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  RHT74_RS03720 (RHT74_03720) pilA 788647..789087 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  RHT74_RS03725 (RHT74_03725) nadC 789291..790184 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHT74_RS03730 (RHT74_03730) ampD 790272..790823 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RHT74_RS03735 (RHT74_03735) ampE 790820..791674 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  RHT74_RS03740 (RHT74_03740) aroP 791717..793087 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=801714 RHT74_RS03720 WP_000360914.1 788647..789087(-) (pilA) [Escherichia coli strain M21816]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=801714 RHT74_RS03720 WP_000360914.1 788647..789087(-) (pilA) [Escherichia coli strain M21816]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363