Detailed information NTG0801566

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: RHT77_RS08170
• Genome accession: NZ_CP133916
• Coordinates: 1702235..1703218 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain M23314
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 1697235..1708218

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHT77_RS08155 (RHT77_08155)	dppA	1698535..1700142 (+)	1608	WP_023155413.1	dipeptide ABC transporter substrate-binding protein DppA	-
RHT77_RS08160 (RHT77_08160)	dppB	1700293..1701312 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
RHT77_RS08165 (RHT77_08165)	dppC	1701322..1702224 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
RHT77_RS08170 (RHT77_08170)	amiE	1702235..1703218 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
RHT77_RS08175 (RHT77_08175)	dppF	1703215..1704219 (+)	1005	WP_000107030.1	dipeptide ABC transporter ATP-binding subunit DppF	-
RHT77_RS08180 (RHT77_08180)	yhjV	1704249..1705520 (-)	1272	WP_001402620.1	aromatic amino acid transport family protein	-
RHT77_RS08185 (RHT77_08185)	ldrD	1705996..1706103 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
RHT77_RS08190 (RHT77_08190)	-	1706479..1706586 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
RHT77_RS08195 (RHT77_08195)	-	1706962..1707069 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=801566 RHT77_RS08170 WP_001196486.1 1702235..1703218(+) (amiE) [Escherichia coli strain M23314]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=801566 RHT77_RS08170 WP_001196486.1 1702235..1703218(+) (amiE) [Escherichia coli strain M23314]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCTGGCGGTATGAGTCAG
CGTGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGTTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGTAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGATGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404