Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RHT80_RS01930 Genome accession   NZ_CP133913
Coordinates   414030..414707 (-) Length   225 a.a.
NCBI ID   WP_000178155.1    Uniprot ID   -
Organism   Escherichia coli strain M23917     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409030..419707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT80_RS01915 (RHT80_01910) chiA 410397..413090 (+) 2694 WP_024250449.1 bifunctional chitinase/lysozyme -
  RHT80_RS01920 (RHT80_01915) bfd 413259..413453 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RHT80_RS01925 (RHT80_01920) bfr 413525..414001 (+) 477 WP_000675504.1 bacterioferritin -
  RHT80_RS01930 (RHT80_01925) pilD 414030..414707 (-) 678 WP_000178155.1 prepilin peptidase GspO Machinery gene
  RHT80_RS01935 (RHT80_01930) gspM 414707..415168 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RHT80_RS01940 (RHT80_01935) gspL 415165..416328 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  RHT80_RS01945 (RHT80_01940) gspK 416343..417326 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RHT80_RS01950 (RHT80_01945) gspJ 417319..417906 (-) 588 WP_000610636.1 type II secretion system minor pseudopilin GspJ -
  RHT80_RS01955 (RHT80_01950) gspI 417899..418276 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RHT80_RS01960 (RHT80_01955) gspH 418273..418782 (-) 510 WP_134778497.1 type II secretion system minor pseudopilin GspH -
  RHT80_RS01965 (RHT80_01960) gspG 418790..419227 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24879.74 Da        Isoelectric Point: 7.6578

>NTDB_id=801465 RHT80_RS01930 WP_000178155.1 414030..414707(-) (pilD) [Escherichia coli strain M23917]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=801465 RHT80_RS01930 WP_000178155.1 414030..414707(-) (pilD) [Escherichia coli strain M23917]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCACCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378