Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHS39_RS14305 Genome accession   NZ_CP133900
Coordinates   3066087..3067811 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain TW01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3061087..3072811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS39_RS14270 (RHS39_14270) - 3061418..3062131 (-) 714 WP_025787806.1 PilW family protein -
  RHS39_RS14275 (RHS39_14275) - 3062132..3062698 (-) 567 WP_025787804.1 type II secretion system protein -
  RHS39_RS14280 (RHS39_14280) - 3062695..3063180 (-) 486 WP_025787802.1 type II secretion system protein -
  RHS39_RS14285 (RHS39_14285) - 3063296..3063529 (-) 234 WP_025787800.1 hypothetical protein -
  RHS39_RS14290 (RHS39_14290) - 3063604..3064095 (-) 492 WP_025787798.1 type II secretion system protein -
  RHS39_RS14295 (RHS39_14295) - 3064390..3064851 (-) 462 WP_024701144.1 hypothetical protein -
  RHS39_RS14300 (RHS39_14300) - 3064855..3066078 (-) 1224 WP_025787794.1 type II secretion system F family protein -
  RHS39_RS14305 (RHS39_14305) pilB 3066087..3067811 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  RHS39_RS14310 (RHS39_14310) - 3067808..3068884 (-) 1077 WP_025541499.1 lipopolysaccharide assembly protein LapB -
  RHS39_RS14315 (RHS39_14315) - 3068881..3069726 (-) 846 WP_005497278.1 ExeA family protein -
  RHS39_RS14320 (RHS39_14320) mshL 3069770..3071413 (-) 1644 WP_020835331.1 pilus (MSHA type) biogenesis protein MshL -
  RHS39_RS14325 (RHS39_14325) - 3071434..3071763 (-) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  RHS39_RS14330 (RHS39_14330) gspM 3071756..3072406 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=801436 RHS39_RS14305 WP_005456306.1 3066087..3067811(-) (pilB) [Vibrio parahaemolyticus strain TW01]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=801436 RHS39_RS14305 WP_005456306.1 3066087..3067811(-) (pilB) [Vibrio parahaemolyticus strain TW01]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTCGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCAGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTTTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTAGCTCGTAATGGTGACACTCTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTAACCGTTGTAAAACTGGTCAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCTTGCGTTTGCGCCAGC
GTGTCGACGGTGTGCTACATGAAACCATTCTGAATGAAGTGAACATCGCTTCAGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGGTCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAAATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGCGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCACTAACCGGCCACTTAGTATTGAGTACGTTGCACACCAACGATGCAGTCGACAGTGCGTTGCG
TATGATCGACATGGGAGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTGGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTTCACAAAGGCGCAGGTTGTCAAAACTGTAACTTGACGGGATATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGCGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCACTGCAAGGTGCTGTGAGCCTAGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361