Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   P0S91_RS10740 Genome accession   NZ_CP119968
Coordinates   2247099..2249099 (-) Length   666 a.a.
NCBI ID   WP_105219985.1    Uniprot ID   A0A6N8FY04
Organism   Gloeocapsopsis dulcis strain AAB1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2242099..2254099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0S91_RS10720 (P0S91_10675) bioB 2242810..2243859 (+) 1050 WP_105219989.1 biotin synthase BioB -
  P0S91_RS10725 (P0S91_10680) - 2243843..2244418 (-) 576 WP_105219988.1 hypothetical protein -
  P0S91_RS10730 (P0S91_10685) - 2244467..2245690 (-) 1224 WP_105219987.1 type II secretion system F family protein -
  P0S91_RS10735 (P0S91_10690) pilT 2245921..2247057 (-) 1137 WP_105219986.1 type IV pilus twitching motility protein PilT Machinery gene
  P0S91_RS10740 (P0S91_10695) pilB/pilB1 2247099..2249099 (-) 2001 WP_105219985.1 GspE/PulE family protein Machinery gene
  P0S91_RS10745 (P0S91_10700) grpE 2249418..2250110 (+) 693 WP_105219984.1 nucleotide exchange factor GrpE -
  P0S91_RS10750 (P0S91_10705) dnaK 2250329..2252305 (+) 1977 WP_105219983.1 molecular chaperone DnaK -
  P0S91_RS10755 (P0S91_10710) dnaJ 2252410..2253540 (+) 1131 WP_105219982.1 molecular chaperone DnaJ -
  P0S91_RS10760 (P0S91_10715) - 2253537..2253797 (+) 261 WP_105219981.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74476.31 Da        Isoelectric Point: 5.1771

>NTDB_id=801278 P0S91_RS10740 WP_105219985.1 2247099..2249099(-) (pilB/pilB1) [Gloeocapsopsis dulcis strain AAB1]
MTYSPSQRRALIVKNDFSPFGNKVIESGYVSNEQMQQALSESRKSKRPITEVLESLTGRQLPPDLLRFYKKQQLFELKIV
YGVEFIDPQINQIAPNQVANLIDTLIPVEICRRFQLVPLYQNTDPPSVLIAMVDPDHLEAQDYLSRILRPKGLGLQRMVM
TPEDFQQLLSKYLDAQVEQQKQRDIQKSVDVQSEIEGLEGFDLQNASDEIEADLGAAIQNAEAAPVIALVNKILVKALQE
KVSDIHIEPQEEHLRVRFRKDGVLRQAFEPLPKKIVPAVVARFKIISELDIAERRMPQDGRIRRVFEGRKVDFRVSTLPS
RYGEKVVLRILDNAATQLGLDKLISDEESLAIVREMASRPFGLLLVTGPTGSGKSTTLYSILAERNEPGVNISTAEDPIE
YSLPGITQVQVIRDKGMDFASILRSFLRQDPDVILVGETRDRETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVE
PFMVSGALLGVVAQRLVRRVCSACRISYSPSSTELARFGLTSSQDAGVSFYKAKTLQPEEITTARSQGELCPECNGVGYK
GRCGVYEVMRVNQRLQNLITEGAPTERIKEAAVEDGMKTLLSYSLDLVRRGYTTLEEVERVTFTDSGLEAELKAKRKSSL
ECQTCSAELQPEWLECPYCMTPRFQD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=801278 P0S91_RS10740 WP_105219985.1 2247099..2249099(-) (pilB/pilB1) [Gloeocapsopsis dulcis strain AAB1]
ATGACTTACTCCCCCTCACAGCGACGTGCCCTGATTGTCAAAAATGACTTTTCTCCTTTCGGCAATAAGGTGATTGAGTC
GGGGTATGTCAGCAATGAACAGATGCAACAAGCTCTGAGTGAAAGTCGTAAATCTAAAAGACCGATAACAGAAGTATTAG
AGTCGCTAACTGGGCGTCAACTACCACCTGACTTATTGAGGTTTTATAAAAAGCAGCAACTCTTTGAACTCAAAATTGTT
TATGGTGTTGAATTTATCGATCCCCAAATCAATCAAATTGCCCCTAATCAAGTTGCAAATTTGATCGACACGCTCATTCC
GGTTGAAATTTGCCGCCGTTTTCAATTAGTTCCATTATATCAAAATACTGACCCTCCTTCGGTATTAATAGCGATGGTCG
ATCCCGATCATCTTGAGGCTCAAGATTATTTGAGTCGAATTCTCCGTCCCAAAGGATTGGGATTGCAACGCATGGTAATG
ACTCCAGAAGACTTCCAGCAGTTGCTTTCCAAATACCTCGACGCACAAGTTGAGCAGCAAAAACAGCGAGACATTCAAAA
ATCAGTTGATGTTCAATCTGAAATAGAAGGCTTAGAGGGTTTTGATTTACAAAATGCCAGTGATGAAATTGAAGCAGATT
TAGGCGCTGCTATTCAAAACGCTGAGGCTGCACCTGTCATTGCTCTAGTTAATAAAATTCTTGTTAAAGCTTTGCAAGAA
AAAGTTTCAGATATTCACATTGAACCGCAAGAAGAACATTTACGGGTTCGCTTTCGCAAAGATGGAGTTCTGCGTCAAGC
ATTTGAGCCACTACCGAAAAAAATTGTGCCGGCTGTTGTTGCTCGATTCAAAATTATCTCGGAACTGGACATTGCTGAGC
GGCGGATGCCTCAAGACGGACGTATTCGACGAGTGTTTGAAGGGCGCAAAGTAGACTTTCGTGTGAGTACTCTACCAAGT
CGCTACGGTGAGAAAGTTGTCCTGCGAATTTTAGATAATGCAGCTACTCAACTTGGTTTAGATAAACTGATTAGCGATGA
AGAGAGTTTAGCAATTGTCCGCGAAATGGCAAGTCGTCCTTTTGGATTACTGTTAGTGACGGGACCAACAGGTTCGGGAA
AATCAACAACCTTATACTCTATCTTGGCAGAACGTAATGAGCCAGGAGTTAATATCAGTACAGCCGAAGATCCGATTGAG
TATTCTTTACCAGGAATTACTCAAGTCCAGGTGATTCGTGACAAAGGTATGGATTTTGCTTCGATTTTGCGGTCATTTCT
GCGGCAAGATCCTGACGTGATTCTTGTCGGTGAGACCCGAGACAGAGAAACTGCTAAAACGGCAATTGAAGCAGCGTTAA
CCGGACACTTAGTCTTAACAACACTCCACACGAATGATGCTGCTGGTGCGATCGCCCGTTTGGATGAAATGGGTGTTGAA
CCTTTTATGGTATCAGGTGCTTTACTTGGTGTTGTTGCTCAGCGTTTGGTTAGACGTGTCTGTTCAGCGTGTCGCATTTC
CTATTCACCTTCGTCTACAGAATTGGCGCGCTTTGGTTTAACAAGTTCGCAAGACGCTGGTGTGTCTTTTTACAAAGCTA
AGACTTTACAGCCTGAAGAAATTACCACGGCGCGCAGTCAAGGCGAGCTTTGTCCAGAGTGTAACGGTGTTGGATACAAA
GGACGTTGTGGGGTATATGAAGTCATGCGCGTTAATCAACGGCTGCAAAATTTAATTACGGAAGGTGCCCCAACCGAACG
CATTAAAGAAGCCGCAGTAGAAGATGGCATGAAAACCCTACTATCTTACAGCCTTGACTTGGTTCGTCGAGGCTACACCA
CACTCGAAGAAGTTGAACGCGTCACATTTACTGATTCTGGCTTAGAAGCAGAACTTAAAGCAAAACGGAAGAGTTCTTTG
GAGTGTCAAACTTGTAGCGCTGAGTTACAACCAGAGTGGTTAGAATGTCCCTACTGTATGACACCGCGTTTTCAAGATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(232-499)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N8FY04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.961

99.249

0.655