Detailed information NTG0801227

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: P3F89_RS21350
• Genome accession: NZ_CP119875
• Coordinates: 4111772..4115497 (-)
• Length: 1241 a.a.
• NCBI ID: WP_309573708.1
• Uniprot ID: -
• Organism: Bacillus tropicus strain T36S-23
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4106772..4120497

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3F89_RS21300 (P3F89_21270)	-	4107605..4107889 (+)	285	WP_098364062.1	hypothetical protein	-
P3F89_RS21305 (P3F89_21275)	-	4107925..4108824 (-)	900	WP_038356988.1	fumarylacetoacetate hydrolase family protein	-
P3F89_RS21310 (P3F89_21280)	-	4109072..4109251 (+)	180	WP_000462845.1	aspartyl-phosphate phosphatase Spo0E family protein	-
P3F89_RS21315 (P3F89_21285)	gerPA	4109348..4109569 (+)	222	WP_001111187.1	spore germination protein GerPA	-
P3F89_RS21320 (P3F89_21290)	gerPB	4109584..4109790 (+)	207	WP_001012508.1	spore germination protein GerPB	-
P3F89_RS21325 (P3F89_21295)	gerPC	4109859..4110473 (+)	615	WP_002194454.1	spore germination protein GerPC	-
P3F89_RS21330 (P3F89_21300)	gerPD	4110480..4110674 (+)	195	WP_001102341.1	spore germination protein GerPD	-
P3F89_RS21335 (P3F89_21305)	-	4110690..4111076 (+)	387	WP_002194453.1	spore germination protein GerPE	-
P3F89_RS21340 (P3F89_21310)	gerPF	4111119..4111334 (+)	216	WP_001141566.1	spore germination protein GerPF	-
P3F89_RS21345 (P3F89_21315)	-	4111472..4111759 (-)	288	WP_002194451.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
P3F89_RS21350 (P3F89_21320)	addA	4111772..4115497 (-)	3726	WP_309573708.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
P3F89_RS21355 (P3F89_21325)	addB	4115494..4119009 (-)	3516	WP_098367907.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
P3F89_RS21360 (P3F89_21330)	lepB	4119126..4119689 (-)	564	WP_002194447.1	signal peptidase I	-
P3F89_RS21365 (P3F89_21335)	-	4119746..4120339 (-)	594	WP_000347516.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142745.10 Da
• Isoelectric Point: 4.8851

>NTDB_id=801227 P3F89_RS21350 WP_309573708.1 4111772..4115497(-) (addA) [Bacillus tropicus strain T36S-23]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEEKPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLINEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVRQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYSASWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFSGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=801227 P3F89_RS21350 WP_309573708.1 4111772..4115497(-) (addA) [Bacillus tropicus strain T36S-23]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAAACCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTAATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATAGCGAATCAAACAGAAAATGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTCCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAGACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGCTATTTAGCCGTAAGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAGCTCGTAAAAGTATTTACCGAGCGTTTCCAAACGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGATTATTCCTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGCACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGTGAAGAAGGCGCAGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCTGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTCGTTATTTTGCTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTGAAATTACAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCCGTGCTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACACTTCG
TGCTCATGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGCGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTACGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCAAGAGCTTTAGGTGAACAAGAAGATGTTGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGTCGCTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGCTTCGGTTCGCAATTTATTGATCCACGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATATGTTGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATACTTCTTGAATTGGGACAAGGAAGCATTCCAGATGAAATTTATGGGTATAGTGCAAGCTGGAAA
GTAGAAGTTGTTGACGGAAACACGTTACTTGCGCCAGAACCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCTCTGCAAAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGAACACGTCCTCGTTTTATGGAGAAAAAAGGTTTAACGTACGCAGAGCGTGG
AACCGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTTGAACAAGCAGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGGAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCTCAGGTGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.828	100	0.538